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| [[Image:2b4k.gif|left|200px]] | | {{Seed}} |
| | [[Image:2b4k.png|left|200px]] |
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| {{STRUCTURE_2b4k| PDB=2b4k | SCENE= }} | | {{STRUCTURE_2b4k| PDB=2b4k | SCENE= }} |
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| '''Acetobacter turbidans alpha-amino acid ester hydrolase complexed with phenylglycine'''
| | ===Acetobacter turbidans alpha-amino acid ester hydrolase complexed with phenylglycine=== |
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| ==Overview==
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| The alpha-amino acid ester hydrolase (AEH) from Acetobacter turbidans is a bacterial enzyme catalyzing the hydrolysis and synthesis of beta-lactam antibiotics. The crystal structures of the native enzyme, both unliganded and in complex with the hydrolysis product D-phenylglycine are reported, as well as the structures of an inactive mutant (S205A) complexed with the substrate ampicillin, and an active site mutant (Y206A) with an increased tendency to catalyze antibiotic production rather than hydrolysis. The structure of the native enzyme shows an acyl binding pocket, in which D-phenylglycine binds, and an additional space that is large enough to accommodate the beta-lactam moiety of an antibiotic. In the S205A mutant, ampicillin binds in this pocket in a non-productive manner, making extensive contacts with the side chain of Tyr(112), which also participates in oxyanion hole formation. In the Y206A mutant, the Tyr(112) side chain has moved with its hydroxyl group toward the catalytic serine. Because this changes the properties of the beta-lactam binding site, this could explain the increased beta-lactam transferase activity of this mutant.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16377627}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16377627 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16377627}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Wybenga, G.]] | | [[Category: Wybenga, G.]] |
| [[Category: Alpha-beta hydrolase]] | | [[Category: Alpha-beta hydrolase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:50:53 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:05:16 2008'' |