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| {{STRUCTURE_2ag1| PDB=2ag1 | SCENE= }} | | {{STRUCTURE_2ag1| PDB=2ag1 | SCENE= }} |
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| '''Crystal structure of Benzaldehyde lyase (BAL)- SeMet'''
| | ===Crystal structure of Benzaldehyde lyase (BAL)- SeMet=== |
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| ==Overview==
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| Pseudomonas fluorescens is able to grow on R-benzoin as the sole carbon and energy source because it harbours the enzyme benzaldehyde lyase that cleaves the acyloin linkage using thiamine diphosphate (ThDP) as a cofactor. In the reverse reaction, this lyase catalyses the carboligation of two aldehydes with high substrate and stereospecificity. The enzyme structure was determined by X-ray diffraction at 2.6 A resolution. A structure-based comparison with other proteins showed that benzaldehyde lyase belongs to a group of closely related ThDP-dependent enzymes. The ThDP cofactors of these enzymes are fixed at their two ends in separate domains, suspending a comparatively mobile thiazolium ring between them. While the residues binding the two ends of ThDP are well conserved, the lining of the active centre pocket around the thiazolium moiety varies greatly within the group. Accounting for the known reaction chemistry, the natural substrate R-benzoin was modelled unambiguously into the active centre of the reported benzaldehyde lyase. Due to its substrate spectrum and stereospecificity, the enzyme extends the synthetic potential for carboligations appreciably.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16302970}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16302970 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16302970}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Tetramer]] | | [[Category: Tetramer]] |
| [[Category: Thdp dependent fold]] | | [[Category: Thdp dependent fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:00:30 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:57:21 2008'' |