2a5c: Difference between revisions

Revision as of 14:06, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:2a5c.png

Template:STRUCTURE 2a5c

Structure of Avidin in complex with the ligand 8-oxodeoxyadenosineStructure of Avidin in complex with the ligand 8-oxodeoxyadenosine

Template:ABSTRACT PUBMED 16413579

About this StructureAbout this Structure

2A5C is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

Recognition of oxidatively modified bases within the biotin-binding site of avidin., Conners R, Hooley E, Clarke AR, Thomas S, Brady RL, J Mol Biol. 2006 Mar 17;357(1):263-74. Epub 2006 Jan 6. PMID:16413579

Page seeded by OCA on Mon Jul 28 14:06:24 2008

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 {{STRUCTURE_2a5c|  PDB=2a5c  |  SCENE=  }}
-'''Structure of Avidin in complex with the ligand 8-oxodeoxyadenosine'''
+===Structure of Avidin in complex with the ligand 8-oxodeoxyadenosine===
-==Overview==
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-Oxidative damage of DNA results in the formation of many products, including 8-oxodeoxyguanosine, which has been used as a marker to quantify DNA damage. Earlier studies have demonstrated that avidin, a protein prevalent in egg-white and which has high affinity for the vitamin biotin, binds to 8-oxodeoxyguanosine and related bases. In this study, we have determined crystal structures of avidin in complex with 8-oxodeoxyguanosine and 8-oxodeoxyadenosine. In each case, the base is observed to bind within the biotin-binding site of avidin. However, the mode of association between the bases and the protein varies and, unlike in the avidin:biotin complex, complete ordering of the protein in this region does not accompany binding. Fluorescence studies indicate that in solution the individual bases, and a range of oligonucleotides, bind to avidin with micromolar affinity. Only one of the modes of binding observed is consistent with recognition of oxidised purines when incorporated within a DNA oligomer, and from this structure a model is proposed for the selective binding of avidin to DNA containing oxidatively damaged deoxyguanosine. These studies illustrate the molecular basis by which avidin might act as a marker of DNA damage, although the low levels of binding observed are inconsistent with the recognition of oxidised purines forming a major physiological role for avidin.
+The line below this paragraph, {{ABSTRACT_PUBMED_16413579}}, adds the Publication Abstract to the page
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+{{ABSTRACT_PUBMED_16413579}}
 ==About this Structure==
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 [[Category: Damaged dna]]
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