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| [[Image:2a3t.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2a3t| PDB=2a3t | SCENE= }} | | {{STRUCTURE_2a3t| PDB=2a3t | SCENE= }} |
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| '''Cu-containing nitrite reductase'''
| | ===Cu-containing nitrite reductase=== |
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| ==Overview==
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| Nitrite reductase is an enzyme operating in the denitrification pathway which catalyses the conversion of nitrite (NO2(-)) to gaseous nitric oxide (NO). Here, crystal structures of the oxidized and reduced forms of the copper-containing nitrite reductase from Rhodobacter sphaeroides 2.4.3 are presented at 1.74 and 1.85 A resolution, respectively. Whereas the structure of the enzyme is very similar to those of other copper-containing nitrite reductases, folding as a trimer and containing two copper sites per monomer, the structures reported here enable conformational differences between the oxidized and reduced forms of the enzyme to be identified. In the type 1 copper site, a rotational perturbation of the side chain of the copper ligand Met182 occurs upon reduction. At the type 2 copper site, a dual conformation of the catalytic residue His287 is observed in the oxidized structure but is lacking in the reduced structure, such that the interactions of the oxidized type 2 copper ion can be regarded as adopting octahedral geometry. These findings shed light on the structural mechanism of the reduction of a copper-bound nitrite to nitric oxide and water.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16131751}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16131751 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16131751}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Denitrification]] | | [[Category: Denitrification]] |
| [[Category: Nitrite reduction]] | | [[Category: Nitrite reduction]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:33:59 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:59:18 2008'' |