2a3t: Difference between revisions

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{{STRUCTURE_2a3t|  PDB=2a3t  |  SCENE=  }}  
{{STRUCTURE_2a3t|  PDB=2a3t  |  SCENE=  }}  


'''Cu-containing nitrite reductase'''
===Cu-containing nitrite reductase===




==Overview==
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Nitrite reductase is an enzyme operating in the denitrification pathway which catalyses the conversion of nitrite (NO2(-)) to gaseous nitric oxide (NO). Here, crystal structures of the oxidized and reduced forms of the copper-containing nitrite reductase from Rhodobacter sphaeroides 2.4.3 are presented at 1.74 and 1.85 A resolution, respectively. Whereas the structure of the enzyme is very similar to those of other copper-containing nitrite reductases, folding as a trimer and containing two copper sites per monomer, the structures reported here enable conformational differences between the oxidized and reduced forms of the enzyme to be identified. In the type 1 copper site, a rotational perturbation of the side chain of the copper ligand Met182 occurs upon reduction. At the type 2 copper site, a dual conformation of the catalytic residue His287 is observed in the oxidized structure but is lacking in the reduced structure, such that the interactions of the oxidized type 2 copper ion can be regarded as adopting octahedral geometry. These findings shed light on the structural mechanism of the reduction of a copper-bound nitrite to nitric oxide and water.
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==About this Structure==
==About this Structure==
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[[Category: Denitrification]]
[[Category: Denitrification]]
[[Category: Nitrite reduction]]
[[Category: Nitrite reduction]]
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