2cv5: Difference between revisions

Revision as of 17:52, 21 February 2008

Crystal structure of human nucleosome core particle

OverviewOverview

Gene expression in eukaryotes depends upon positioning, mobility and packaging of nucleosomes; thus, we need the detailed information of the human nucleosome core particle (NCP) structure, which could clarify chromatin properties. Here, we report the 2.5 A crystal structure of a human NCP. The overall structure is similar to those of other NCPs reported previously. However, the DNA path of human NCP is remarkably different from that taken within other NCPs with an identical DNA sequence. A comparison of the structural parameters between human and Xenopus laevis DNA reveals that the DNA path of human NCP consecutively shifts by 1 bp in the regions of superhelix axis location -5.0 to -2.0 and 5.0 to 7.0. This alteration of the human DNA path is caused predominantly by tight DNA-DNA contacts within the crystal. It is also likely that the conformational change in the human H2B tail induces the local alteration of the DNA path. In human NCP, the region with the altered DNA path lacks Mn2+ ions and the B-factors of the DNA phosphate groups are substantially high. Therefore, in contrast to the histone octamer, the nucleosomal DNA is sufficiently flexible and mobile and can undergo drastic conformational changes, depending upon the environment.

About this StructureAbout this Structure

2CV5 is a Protein complex structure of sequences from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Alteration of the nucleosomal DNA path in the crystal structure of a human nucleosome core particle., Tsunaka Y, Kajimura N, Tate S, Morikawa K, Nucleic Acids Res. 2005 Jun 10;33(10):3424-34. Print 2005. PMID:15951514

Page seeded by OCA on Thu Feb 21 16:52:44 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:2cv5.gif|left|200px]]<br />
+[[Image:2cv5.gif|left|200px]]<br /><applet load="2cv5" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="2cv5" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="2cv5, resolution 2.5&Aring;" />
 '''Crystal structure of human nucleosome core particle'''<br />
 ==Overview==
-Gene expression in eukaryotes depends upon positioning, mobility and, packaging of nucleosomes; thus, we need the detailed information of the, human nucleosome core particle (NCP) structure, which could clarify, chromatin properties. Here, we report the 2.5 A crystal structure of a, human NCP. The overall structure is similar to those of other NCPs, reported previously. However, the DNA path of human NCP is remarkably, different from that taken within other NCPs with an identical DNA, sequence. A comparison of the structural parameters between human and, Xenopus laevis DNA reveals that the DNA path of human NCP consecutively, shifts by 1 bp in the regions of superhelix axis location -5.0 to -2.0 and, 5.0 to 7.0. This alteration of the human DNA path is caused predominantly, by tight DNA-DNA contacts within the crystal. It is also likely that the, conformational change in the human H2B tail induces the local alteration, of the DNA path. In human NCP, the region with the altered DNA path lacks, Mn2+ ions and the B-factors of the DNA phosphate groups are substantially, high. Therefore, in contrast to the histone octamer, the nucleosomal DNA, is sufficiently flexible and mobile and can undergo drastic conformational, changes, depending upon the environment.
+Gene expression in eukaryotes depends upon positioning, mobility and packaging of nucleosomes; thus, we need the detailed information of the human nucleosome core particle (NCP) structure, which could clarify chromatin properties. Here, we report the 2.5 A crystal structure of a human NCP. The overall structure is similar to those of other NCPs reported previously. However, the DNA path of human NCP is remarkably different from that taken within other NCPs with an identical DNA sequence. A comparison of the structural parameters between human and Xenopus laevis DNA reveals that the DNA path of human NCP consecutively shifts by 1 bp in the regions of superhelix axis location -5.0 to -2.0 and 5.0 to 7.0. This alteration of the human DNA path is caused predominantly by tight DNA-DNA contacts within the crystal. It is also likely that the conformational change in the human H2B tail induces the local alteration of the DNA path. In human NCP, the region with the altered DNA path lacks Mn2+ ions and the B-factors of the DNA phosphate groups are substantially high. Therefore, in contrast to the histone octamer, the nucleosomal DNA is sufficiently flexible and mobile and can undergo drastic conformational changes, depending upon the environment.
 ==About this Structure==
-CV5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MN and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CV5 OCA].
+CV5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CV5 OCA].
 ==Reference==
@@ Line 25: / Line 24: @@
 [[Category: x-ray crystallography]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 21:24:19 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:52:44 2008''