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| [[Image:1z1v.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1z1v| PDB=1z1v | SCENE= }} | | {{STRUCTURE_1z1v| PDB=1z1v | SCENE= }} |
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| '''NMR structure of the Saccharomyces cerevisiae Ste50 SAM domain'''
| | ===NMR structure of the Saccharomyces cerevisiae Ste50 SAM domain=== |
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| ==Overview==
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| In Saccharomyces cerevisiae, signal transduction through pathways governing mating, osmoregulation, and nitrogen starvation depends upon a direct interaction between the sterile alpha motif (SAM) domains of the Ste11 mitogen-activated protein kinase kinase kinase (MAPKKK) and its regulator Ste50. Previously, we solved the NMR structure of the SAM domain from Ste11 and identified two mutants that diminished binding to the Ste50 SAM domain. Building upon the Ste11 study, we present the NMR structure of the monomeric Ste50 SAM domain and a series of mutants bearing substitutions at surface-exposed hydrophobic amino acid residues. The mid-loop (ML) region of Ste11-SAM, defined by helices H3 and H4 and the end-helix (EH) region of Ste50-SAM, defined by helix H5, were sensitive to substitution, indicating that these two surfaces contribute to the high-affinity interaction. The combination of two mutants, Ste11-SAM-L72R and Ste50-SAM-L69R, formed a high-affinity heterodimer unencumbered by competing homotypic interactions that had prevented earlier NMR studies of the wild-type complex. Yeast bearing mutations that prevented the heterotypic Ste11-Ste50 association in vitro presented signaling defects in the mating and high-osmolarity growth pathways.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16337230}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16337230 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16337230}} |
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| ==About this Structure== | | ==About this Structure== |
| 1Z1V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1V OCA]. | | 1Z1V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z1V OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: All helix protein]] | | [[Category: All helix protein]] |
| [[Category: Sam domain]] | | [[Category: Sam domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:05:09 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:24:53 2008'' |