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| {{STRUCTURE_1yu8| PDB=1yu8 | SCENE= }} | | {{STRUCTURE_1yu8| PDB=1yu8 | SCENE= }} |
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| '''Crystal Structure of the R37A Mutant of Villin Headpiece'''
| | ===Crystal Structure of the R37A Mutant of Villin Headpiece=== |
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| ==Overview==
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| Villin-type headpiece domains are approximately 70 amino acid modular motifs found at the C terminus of a variety of actin cytoskeleton-associated proteins. The headpiece domain of villin, a protein found in the actin bundles of the brush border epithelium, is of interest both as a compact F-actin binding domain and as a model folded protein. We have determined the high-resolution crystal structures of chicken villin headpiece (HP67) at 1.4 A resolution as well as two mutants, R37A and W64Y, at 1.45 and 1.5 A resolution, respectively. Replacement of R37 causes a 5-fold reduction in F-actin binding affinity in sedimentation assays. Replacement of W64 results in a much more drastic reduction in F-actin binding affinity without significant changes in headpiece structure or stability. The detailed comparison of these crystal structures with each other and to our previously determined NMR structures of HP67 and the 35-residue autonomously folding subdomain in villin headpiece, HP35, provides the details of the headpiece fold and further defines the F-actin binding site of villin-type headpiece domains.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16142894}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16142894 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16142894}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: 3-10 helix]] | | [[Category: 3-10 helix]] |
| [[Category: Alpha helix]] | | [[Category: Alpha helix]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:47:45 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:56:25 2008'' |