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| {{STRUCTURE_1ymo| PDB=1ymo | SCENE= }} | | {{STRUCTURE_1ymo| PDB=1ymo | SCENE= }} |
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| '''Solution structure of the P2b-P3 pseudoknot from human telomerase RNA'''
| | ===Solution structure of the P2b-P3 pseudoknot from human telomerase RNA=== |
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| ==Overview==
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| Human telomerase contains a 451 nt RNA (hTR) and several proteins, including a specialized reverse transcriptase (hTERT). The 5' half of hTR comprises the pseudoknot (core) domain, which includes the RNA template for telomere synthesis and a highly conserved pseudoknot that is required for telomerase activity. The solution structure of this essential pseudoknot, presented here, reveals an extended triple helix surrounding the helical junction. The network of tertiary interactions explains the phylogenetic sequence conservation and existing human and mouse TR functional studies as well as mutations linked to disease. Thermodynamic stability, dimerization potential, and telomerase activity of mutant RNAs that alter the tertiary contacts were investigated. Telomerase activity is strongly correlated with tertiary structure stability, whereas there is no correlation with dimerization potential of the pseudoknot. These studies reveal that a conserved pseudoknot tertiary structure is required for telomerase activity.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15749017}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15749017 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15749017}} |
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| ==About this Structure== | | ==About this Structure== |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YMO OCA]. | | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YMO OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Pseudoknot]] | | [[Category: Pseudoknot]] |
| [[Category: Tertiary structure]] | | [[Category: Tertiary structure]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:31:00 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:00:39 2008'' |