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| [[Image:1yd8.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1yd8| PDB=1yd8 | SCENE= }} | | {{STRUCTURE_1yd8| PDB=1yd8 | SCENE= }} |
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| '''COMPLEX OF HUMAN GGA3 GAT DOMAIN AND UBIQUITIN'''
| | ===COMPLEX OF HUMAN GGA3 GAT DOMAIN AND UBIQUITIN=== |
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| ==Overview==
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| The Golgi-localized, gamma-ear-containing, Arf (ADP-ribosylation factor)-binding (GGA) proteins are clathrin adaptors that mediate the sorting of transmembrane-cargo molecules at the trans-Golgi network and endosomes. Cargo proteins can be directed into the GGA pathway by at least two different types of sorting signals: acidic cluster-dileucine motifs and covalent modification by ubiquitin. The latter modification is recognized by the GGAs through binding to their GAT [GGA and TOM (target of Myb)] domain. Here we report the crystal structure of the GAT domain of human GGA3 in a 1:1 complex with ubiquitin at 2.8-A resolution. Ubiquitin binds to a hydrophobic and acidic patch on helices alpha1 and alpha2 of the GAT three-helix bundle that includes Asn-223, Leu-227, Glu-230, Met-231, Asp-244, Glu-246, Leu-247, Glu-250, and Leu-251. The GAT-binding surface on ubiquitin is a hydrophobic patch centered on Ile-44 that is also responsible for binding most other ubiquitin effectors. The ubiquitin-binding site observed in the crystal is distinct from the Rabaptin-5-binding site on helices alpha2 and alpha3 of the GAT domain. Mutational analysis and modeling of the ubiquitin-Rabaptin-5-GAT ternary complex indicates that ubiquitin and Rabaptin-5 can bind to the GAT domain at two different sites without any steric conflict. This ability highlights the GAT domain as a hub for interactions with multiple partners in trafficking. | | The line below this paragraph, {{ABSTRACT_PUBMED_15701688}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15701688 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15701688}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Protein transport;]] | | [[Category: Protein transport;]] |
| [[Category: Trafficking]] | | [[Category: Trafficking]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:10:42 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:03:05 2008'' |