2bf8: Difference between revisions
New page: left|200px<br /> <applet load="2bf8" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bf8, resolution 2.30Å" /> '''CRYSTAL STRUCTURE O... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2bf8.gif|left|200px]]<br /> | [[Image:2bf8.gif|left|200px]]<br /><applet load="2bf8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2bf8" size=" | |||
caption="2bf8, resolution 2.30Å" /> | caption="2bf8, resolution 2.30Å" /> | ||
'''CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K'''<br /> | '''CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K'''<br /> | ||
==Overview== | ==Overview== | ||
Post-translational modification with small ubiquitin-related modifier | Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets. | ||
==Disease== | ==Disease== | ||
| Line 11: | Line 10: | ||
==About this Structure== | ==About this Structure== | ||
2BF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http:// | 2BF8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BF8 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 19: | Line 18: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Ubiquitin--protein ligase]] | [[Category: Ubiquitin--protein ligase]] | ||
[[Category: Dijk, W | [[Category: Dijk, W J.Van.]] | ||
[[Category: Jentsch, S.]] | [[Category: Jentsch, S.]] | ||
[[Category: Knipscheer, P.]] | [[Category: Knipscheer, P.]] | ||
| Line 25: | Line 24: | ||
[[Category: Melchior, F.]] | [[Category: Melchior, F.]] | ||
[[Category: Oberhofer, E.]] | [[Category: Oberhofer, E.]] | ||
[[Category: Olsen, J | [[Category: Olsen, J Velgaard.]] | ||
[[Category: Pichler, A.]] | [[Category: Pichler, A.]] | ||
[[Category: SPINE, Structural | [[Category: SPINE, Structural Proteomics in Europe.]] | ||
[[Category: Sixma, T | [[Category: Sixma, T K.]] | ||
[[Category: e2 ubiquitin conjugating enzyme]] | [[Category: e2 ubiquitin conjugating enzyme]] | ||
[[Category: e2-25k]] | [[Category: e2-25k]] | ||
| Line 38: | Line 37: | ||
[[Category: sumo-target structure]] | [[Category: sumo-target structure]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:37:12 2008'' | ||
Revision as of 17:37, 21 February 2008
|
CRYSTAL STRUCTURE OF SUMO MODIFIED UBIQUITIN CONJUGATING ENZYME E2-25K
OverviewOverview
Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets.
DiseaseDisease
Known diseases associated with this structure: Orofacial cleft 10 OMIM:[601912]
About this StructureAbout this Structure
2BF8 is a Protein complex structure of sequences from Bos taurus and Homo sapiens. Active as Ubiquitin--protein ligase, with EC number 6.3.2.19 Full crystallographic information is available from OCA.
ReferenceReference
SUMO modification of the ubiquitin-conjugating enzyme E2-25K., Pichler A, Knipscheer P, Oberhofer E, van Dijk WJ, Korner R, Olsen JV, Jentsch S, Melchior F, Sixma TK, Nat Struct Mol Biol. 2005 Mar;12(3):264-9. Epub 2005 Feb 20. PMID:15723079
Page seeded by OCA on Thu Feb 21 16:37:12 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Bos taurus
- Homo sapiens
- Protein complex
- Ubiquitin--protein ligase
- Dijk, W J.Van.
- Jentsch, S.
- Knipscheer, P.
- Korner, R.
- Melchior, F.
- Oberhofer, E.
- Olsen, J Velgaard.
- Pichler, A.
- SPINE, Structural Proteomics in Europe.
- Sixma, T K.
- E2 ubiquitin conjugating enzyme
- E2-25k
- Ligase
- Spine
- Structural genomics
- Structural proteomics in europe
- Sumo
- Sumo-target structure