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| '''Hydrogen Bonding in Human Manganese Superoxide Dismutase containing 3-Fluorotyrosine'''
| | ===Hydrogen Bonding in Human Manganese Superoxide Dismutase containing 3-Fluorotyrosine=== |
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| ==Overview==
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| Incorporation of 3-fluorotyrosine and site-specific mutagenesis has been utilized with Fourier transform infrared (FTIR) spectroscopy and x-ray crystallography to elucidate active-site structure and the role of an active-site residue Tyr34 in human manganese superoxide dismutase (MnSOD). Calculated harmonic frequencies at the B3LYP/6-31G** level of theory for L-tyrosine and its 3-fluorine substituted analog are compared to experimental frequencies for vibrational mode assignments. Each of the nine tyrosine residues in each of the four subunits of the homotetramer of human MnSOD was replaced with 3-fluorotyrosine. The crystal structures of the unfluorinated and fluorinated wild-type MnSOD are nearly superimposable with the root mean-square deviation for 198 alpha-carbon atoms at 0.3 A. The FTIR data show distinct vibrational modes arising from 3-fluorotyrosine in MnSOD. Comparison of spectra for wild-type and Y34F MnSOD showed that the phenolic hydroxyl of Tyr34 is hydrogen bonded, acting as a proton donor in the active site. Comparison with crystal structures demonstrates that the hydroxyl of Tyr34 is a hydrogen bond donor to an adjacent water molecule; this confirms the participation of Tyr34 in a network of residues and water molecules that extends from the active site to the adjacent subunit.
| | The line below this paragraph, {{ABSTRACT_PUBMED_16150974}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 16150974 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_16150974}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Manganese superoxide dismutase]] | | [[Category: Manganese superoxide dismutase]] |
| [[Category: Mnsod]] | | [[Category: Mnsod]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:53:10 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:11:06 2008'' |