2a19: Difference between revisions
New page: left|200px<br /> <applet load="2a19" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a19, resolution 2.50Å" /> '''PKR kinase domain- ... |
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[[Image:2a19.gif|left|200px]]<br /> | [[Image:2a19.gif|left|200px]]<br /><applet load="2a19" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="2a19, resolution 2.50Å" /> | caption="2a19, resolution 2.50Å" /> | ||
'''PKR kinase domain- eIF2alpha- AMP-PNP complex.'''<br /> | '''PKR kinase domain- eIF2alpha- AMP-PNP complex.'''<br /> | ||
==Overview== | ==Overview== | ||
In response to binding viral double-stranded RNA byproducts within a cell, the RNA-dependent protein kinase PKR phosphorylates the alpha subunit of | In response to binding viral double-stranded RNA byproducts within a cell, the RNA-dependent protein kinase PKR phosphorylates the alpha subunit of the translation initiation factor eIF2 on a regulatory site, Ser51. This triggers the general shutdown of protein synthesis and inhibition of viral propagation. To understand the basis for substrate recognition by and the regulation of PKR, we determined X-ray crystal structures of the catalytic domain of PKR in complex with eIF2alpha. The structures reveal that eIF2alpha binds to the C-terminal catalytic lobe while catalytic-domain dimerization is mediated by the N-terminal lobe. In addition to inducing a local unfolding of the Ser51 acceptor site in eIF2alpha, its mode of binding to PKR affords the Ser51 site full access to the catalytic cleft of PKR. The generality and implications of the structural mechanisms uncovered for PKR to the larger family of four human eIF2alpha protein kinases are discussed. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
2A19 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG, PO4 and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2A19 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=ANP:'>ANP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A19 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Dar, A | [[Category: Dar, A C.]] | ||
[[Category: Dever, T | [[Category: Dever, T E.]] | ||
[[Category: Sicheri, F.]] | [[Category: Sicheri, F.]] | ||
[[Category: ANP]] | [[Category: ANP]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:22:35 2008'' | ||
Revision as of 17:22, 21 February 2008
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PKR kinase domain- eIF2alpha- AMP-PNP complex.
OverviewOverview
In response to binding viral double-stranded RNA byproducts within a cell, the RNA-dependent protein kinase PKR phosphorylates the alpha subunit of the translation initiation factor eIF2 on a regulatory site, Ser51. This triggers the general shutdown of protein synthesis and inhibition of viral propagation. To understand the basis for substrate recognition by and the regulation of PKR, we determined X-ray crystal structures of the catalytic domain of PKR in complex with eIF2alpha. The structures reveal that eIF2alpha binds to the C-terminal catalytic lobe while catalytic-domain dimerization is mediated by the N-terminal lobe. In addition to inducing a local unfolding of the Ser51 acceptor site in eIF2alpha, its mode of binding to PKR affords the Ser51 site full access to the catalytic cleft of PKR. The generality and implications of the structural mechanisms uncovered for PKR to the larger family of four human eIF2alpha protein kinases are discussed.
DiseaseDisease
Known disease associated with this structure: Kallmann syndrome 3 OMIM:[607123]
About this StructureAbout this Structure
2A19 is a Protein complex structure of sequences from Homo sapiens and Saccharomyces cerevisiae with , and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Higher-order substrate recognition of eIF2alpha by the RNA-dependent protein kinase PKR., Dar AC, Dever TE, Sicheri F, Cell. 2005 Sep 23;122(6):887-900. PMID:16179258
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