1gy3: Difference between revisions
New page: left|200px<br /> <applet load="1gy3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gy3, resolution 2.7Å" /> '''PCDK2/CYCLIN A IN CO... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
1GY3 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MG, NO3, ATP and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GY3 OCA]]. | 1GY3 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MG, NO3, ATP and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GY3 OCA]]. | ||
==Reference== | ==Reference== | ||
| Line 27: | Line 27: | ||
[[Category: thr160-phospho-cyclin dependent protein kinase 2 in association with cyclin a]] | [[Category: thr160-phospho-cyclin dependent protein kinase 2 in association with cyclin a]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:59:15 2007'' | ||
Revision as of 12:54, 30 October 2007
|
PCDK2/CYCLIN A IN COMPLEX WITH MGADP, NITRATE AND PEPTIDE SUBSTRATE
OverviewOverview
Eukaryotic protein kinases catalyze the phosphoryl transfer of the, gamma-phosphate of ATP to the serine, threonine, or tyrosine residue of, protein substrates. The catalytic mechanism of phospho-CDK2/cyclin A, (pCDK2/cyclin A) has been probed with structural and kinetic studies using, the trigonal NO(3)(-) ion, which can be viewed as a mimic of the, metaphosphate transition state. The crystal structure of pCDK2/cyclin A in, complex with Mg(2+)ADP, nitrate, and a heptapeptide substrate has been, determined at 2.7 A. The nitrate ion is located between the beta-phosphate, of ADP and the hydroxyl group of the serine residue of the substrate. In, one molecule of the asymmetric unit, the nitrate is close to the, beta-phosphate of ADP (distance from the nitrate nitrogen to the nearest, ... [(full description)]
About this StructureAbout this Structure
1GY3 is a [Protein complex] structure of sequences from [Homo sapiens] with MG, NO3, ATP and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
Structural studies on phospho-CDK2/cyclin A bound to nitrate, a transition state analogue: implications for the protein kinase mechanism., Cook A, Lowe ED, Chrysina ED, Skamnaki VT, Oikonomakos NG, Johnson LN, Biochemistry. 2002 Jun 11;41(23):7301-11. PMID:12044161
Page seeded by OCA on Tue Oct 30 11:59:15 2007