|
|
| Line 1: |
Line 1: |
| [[Image:1utb.gif|left|200px]] | | {{Seed}} |
| | [[Image:1utb.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1utb| PDB=1utb | SCENE= }} | | {{STRUCTURE_1utb| PDB=1utb | SCENE= }} |
|
| |
|
| '''DNTR FROM BURKHOLDERIA SP. STRAIN DNT'''
| | ===DNTR FROM BURKHOLDERIA SP. STRAIN DNT=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| The transcriptional regulator DntR, a member of the LysR family, is a central element in a prototype bacterial cell-based biosensor for the detection of hazardous contamination of soil and groundwater by dinitrotoluenes. To optimise the sensitivity of the biosensor for such compounds we have chosen a rational design of the inducer-binding cavity based on knowledge of the three-dimensional structure of DntR. We report two crystal structures of DntR with acetate (resolution 2.6 angstroms) and thiocyanate (resolution 2.3 angstroms), respectively, occupying the inducer-binding cavity. These structures allow for the construction of models of DntR in complex with salicylate (Kd approximately or = 4 microM) and 2,4-dinitrotoluene that provide a basis for the design of mutant DntR with enhanced specificity for dinitrotoluenes. In both crystal structures DntR crystallises as a homodimer with a "head-to-tail" arrangement of monomers in the asymmetric unit. Analysis of the crystal structure has allowed the building of a full-length model of DntR in its biologically active homotetrameric form consisting of two "head-to-head" dimers. The implications of this model for the mechanism of transcription regulation by LysR proteins are discussed. | | The line below this paragraph, {{ABSTRACT_PUBMED_15210343}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15210343 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_15210343}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 30: |
Line 34: |
| [[Category: Lysr]] | | [[Category: Lysr]] |
| [[Category: Transcriptional regulator]] | | [[Category: Transcriptional regulator]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:39:37 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:12:48 2008'' |