1gx5: Difference between revisions
New page: left|200px<br /> <applet load="1gx5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gx5, resolution 1.70Å" /> '''HEPATITIS C VIRUS R... |
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==About this Structure== | ==About this Structure== | ||
1GX5 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Hepatitis_c_virus_genotype_1b_(isolate_bk) Hepatitis c virus genotype 1b (isolate bk)]] with MN and GTP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GX5 OCA]]. | 1GX5 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Hepatitis_c_virus_genotype_1b_(isolate_bk) Hepatitis c virus genotype 1b (isolate bk)]] with MN and GTP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48]]. Structure known Active Site: C. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GX5 OCA]]. | ||
==Reference== | ==Reference== | ||
Structural analysis of the hepatitis C virus RNA polymerase in complex with ribonucleotides., Bressanelli S, Tomei L, Rey FA, De Francesco R, J Virol. 2002 Apr;76(7):3482-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11884572 11884572] | Structural analysis of the hepatitis C virus RNA polymerase in complex with ribonucleotides., Bressanelli S, Tomei L, Rey FA, De Francesco R, J Virol. 2002 Apr;76(7):3482-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11884572 11884572] | ||
[[Category: Hepatitis c virus genotype 1b (isolate bk)]] | [[Category: Hepatitis c virus genotype 1b (isolate bk)]] | ||
[[Category: RNA-directed RNA polymerase]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bressanelli, S.]] | [[Category: Bressanelli, S.]] | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
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Revision as of 12:53, 30 October 2007
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HEPATITIS C VIRUS RNA POLYMERASE IN COMPLEX WITH GTP AND MANGANESE
OverviewOverview
We report here the results of a systematic high-resolution X-ray, crystallographic analysis of complexes of the hepatitis C virus (HCV) RNA, polymerase with ribonucleoside triphosphates (rNTPs) and divalent metal, ions. An unexpected observation revealed by this study is the existence of, a specific rGTP binding site in a shallow pocket at the molecular surface, of the enzyme, 30 A away from the catalytic site. This previously, unidentified rGTP pocket, which lies at the interface between fingers and, thumb, may be an allosteric regulatory site and could play a role in, allowing alternative interactions between the two domains during a, possible conformational change of the enzyme required for efficient, initiation. The electron density map at 1.7-A resolution clearly shows the, mode of ... [(full description)]
About this StructureAbout this Structure
1GX5 is a [Single protein] structure of sequence from [Hepatitis c virus genotype 1b (isolate bk)] with MN and GTP as [ligands]. Active as [RNA-directed RNA polymerase], with EC number [2.7.7.48]. Structure known Active Site: C. Full crystallographic information is available from [OCA].
ReferenceReference
Structural analysis of the hepatitis C virus RNA polymerase in complex with ribonucleotides., Bressanelli S, Tomei L, Rey FA, De Francesco R, J Virol. 2002 Apr;76(7):3482-92. PMID:11884572
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