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| [[Image:1u0f.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1u0f| PDB=1u0f | SCENE= }} | | {{STRUCTURE_1u0f| PDB=1u0f | SCENE= }} |
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| '''Crystal structure of mouse phosphoglucose isomerase in complex with glucose 6-phosphate'''
| | ===Crystal structure of mouse phosphoglucose isomerase in complex with glucose 6-phosphate=== |
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| ==Overview==
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| Phosphoglucose isomerase (PGI) is an enzyme of glycolysis that interconverts glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P) but, outside the cell, is a multifunctional cytokine. High-resolution crystal structures of the enzyme from mouse have been determined in native form and in complex with the inhibitor erythrose 4-phosphate, and with the substrate glucose 6-phosphate. In the substrate-bound structure, the glucose sugar is observed in both straight-chain and ring forms. This structure supports a specific role for Lys518 in enzyme-catalyzed ring opening and we present a "push-pull" mechanism in which His388 breaks the O5-C1 bond by donating a proton to the ring oxygen atom and, simultaneously, Lys518 abstracts a proton from the C1 hydroxyl group. The reverse occurs in ring closure. The transition from ring form to straight-chain substrate is achieved through rotation of the C3-C4 bond, which brings the C1-C2 region into close proximity to Glu357, the base catalyst for the isomerization step. The structure with G6P also explains the specificity of PGI for glucose 6-phosphate over mannose 6-isomerase (M6P). To isomerize M6P to F6P requires a rotation of its C2-C3 bond but in PGI this is sterically blocked by Gln511.
| | The line below this paragraph, {{ABSTRACT_PUBMED_15342241}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 15342241 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_15342241}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Aldose-ketose isomerase]] | | [[Category: Aldose-ketose isomerase]] |
| [[Category: Dimer]] | | [[Category: Dimer]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:35:45 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:05:43 2008'' |