Endonuclease: Difference between revisions
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*'''ENN-II''' operates on overlapping restriction site and cleavage pattern. | *'''ENN-II''' operates on overlapping restriction site and cleavage pattern. | ||
*'''ENN-III''' is a bifunctional DNA glycosylase that removes oxidized pyrimidines from DNA<ref>PMID:30959406</ref>. | *'''ENN-III''' is a bifunctional DNA glycosylase that removes oxidized pyrimidines from DNA<ref>PMID:30959406</ref>. | ||
*'''ENN-IV''' cleaves the DNA backbone 5' of AP sites<ref>PMID:10458614</ref>. | |||
Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN<ref>PMID:12483517</ref>.<br /> | Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN<ref>PMID:12483517</ref>.<br /> | ||
The '''Cas ENN''' proteins are part of '''CRISPR/Cas''' prokaryotic immune system which confers protection from foreign genetic elements like viruses. The '''CRISPR''' ('''C'''lustered '''R'''egularly '''I'''nterspersed '''S'''hort '''P'''alindromic '''R'''epeats) are DNA loci which are found in ca. 40% of the bacteria. The CRISPR/Cas system is being used lately as gene editing tool<ref>PMID:20056882</ref>. For more details see<br /> | The '''Cas ENN''' proteins are part of '''CRISPR/Cas''' prokaryotic immune system which confers protection from foreign genetic elements like viruses. The '''CRISPR''' ('''C'''lustered '''R'''egularly '''I'''nterspersed '''S'''hort '''P'''alindromic '''R'''epeats) are DNA loci which are found in ca. 40% of the bacteria. The CRISPR/Cas system is being used lately as gene editing tool<ref>PMID:20056882</ref>. For more details see<br /> | ||