|
|
| Line 1: |
Line 1: |
| [[Image:1sip.gif|left|200px]] | | {{Seed}} |
| | [[Image:1sip.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1sip| PDB=1sip | SCENE= }} | | {{STRUCTURE_1sip| PDB=1sip | SCENE= }} |
|
| |
|
| '''ALTERNATIVE NATIVE FLAP CONFORMATION REVEALED BY 2.3 ANGSTROMS RESOLUTION STRUCTURE OF SIV PROTEINASE'''
| | ===ALTERNATIVE NATIVE FLAP CONFORMATION REVEALED BY 2.3 ANGSTROMS RESOLUTION STRUCTURE OF SIV PROTEINASE=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| A large conformational change is observed between HIV-1 proteinase in the ligand-free state and in complexes with transition-state inhibitors. Crystal structures of this enzyme have either the flaps open for the native or ligand-free enzyme or the flaps closed for peptidomimetic ligand-bound enzyme. We describe the structure of native recombinant SIV proteinase which like other retroviral proteinases crystallizes as a perfect 2-fold symmetric dimer but in a different crystal packing arrangement. In contrast to HIV-1 PR we show that SIV proteinase in the ligand-free state adopts the closed flaps conformation, demonstrating that ligand binding is not a prerequisite for the closed flaps conformation. The catalytic water was clearly observed between the two aspartates which were not perfectly co-planar, and in this structure the active site cleft is more restricted than for either inhibitor bound or ligand-free HIV-1 proteinase. Accommodation of two bulkier side-chains in the simian enzyme core has resulted in a more exposed N terminus than for HIV-1 PR which we predict could enhance autocatalytic cleavage at the N terminus.
| | The line below this paragraph, {{ABSTRACT_PUBMED_8196050}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 8196050 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_8196050}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 23: |
Line 27: |
| [[Category: Single protein]] | | [[Category: Single protein]] |
| [[Category: Wilderspin, A F.]] | | [[Category: Wilderspin, A F.]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:44:58 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:57:27 2008'' |