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| {{STRUCTURE_1s0d| PDB=1s0d | SCENE= }} | | {{STRUCTURE_1s0d| PDB=1s0d | SCENE= }} |
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| '''Crystal structure of botulinum neurotoxin type B at pH 5.5'''
| | ===Crystal structure of botulinum neurotoxin type B at pH 5.5=== |
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| ==Overview==
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| Clostridium botulinum neurotoxins are the most potent toxins to humans and cause paralysis by blocking neurotransmitter release at the presynaptic nerve terminals. The toxicity involves four steps, viz., binding to neuronal cells, internalization, translocation, and catalytic activity. While the catalytic activity is a zinc endopeptidase activity on the SNARE complex proteins, the translocation is believed to be a pH-dependent process allowing the translocation domain to change its conformation to penetrate the endosomal membrane. Here, we report the crystal structures of botulinum neurotoxin type B at various pHs and of an apo form of the neurotoxin, and discuss the role of metal ions and the effect of pH variation in the biological activity. Except for the perturbation of a few side chains, the conformation of the catalytic domain is unchanged in the zinc-depleted apotoxin, suggesting that zinc's role is catalytic. We have also identified two calcium ions in the molecule and present biochemical evidence to show that they play a role in the translocation of the light chain through the membrane.
| | The line below this paragraph, {{ABSTRACT_PUBMED_14979717}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 14979717 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_14979717}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Neurotoxin]] | | [[Category: Neurotoxin]] |
| [[Category: Ph]] | | [[Category: Ph]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:08:22 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:15:15 2008'' |