1rwj: Difference between revisions

Revision as of 02:31, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1rwj.png

Template:STRUCTURE 1rwj

c7-type three-heme cytochrome domainc7-type three-heme cytochrome domain

Template:ABSTRACT PUBMED 15133162

About this StructureAbout this Structure

1RWJ is a Single protein structure of sequence from Geobacter sulfurreducens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a novel c7-type three-heme cytochrome domain from a multidomain cytochrome c polymer., Pokkuluri PR, Londer YY, Duke NE, Erickson J, Pessanha M, Salgueiro CA, Schiffer M, Protein Sci. 2004 Jun;13(6):1684-92. Epub 2004 May 7. PMID:15133162

Family of cytochrome c7-type proteins from Geobacter sulfurreducens: structure of one cytochrome c7 at 1.45 A resolution., Pokkuluri PR, Londer YY, Duke NE, Long WC, Schiffer M, Biochemistry. 2004 Feb 3;43(4):849-59. PMID:14744127

Page seeded by OCA on Mon Jul 28 02:31:51 2008

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OCA

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-[[Image:1rwj.gif|left|200px]]
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 {{STRUCTURE_1rwj|  PDB=1rwj  |  SCENE=  }}
-'''c7-type three-heme cytochrome domain'''
+===c7-type three-heme cytochrome domain===
-==Overview==
+<!--
-The structure of a novel c(7)-type cytochrome domain that has two bishistidine coordinated hemes and one heme with histidine, methionine coordination (where the sixth ligand is a methionine residue) was determined at 1.7 A resolution. This domain is a representative of domains that form three polymers encoded by the Geobacter sulfurreducens genome. Two of these polymers consist of four and one protein of nine c(7)-type domains with a total of 12 and 27 hemes, respectively. Four individual domains (termed A, B, C, and D) from one such multiheme cytochrome c (ORF03300) were cloned and expressed in Escherichia coli. The domain C produced diffraction quality crystals from 2.4 M sodium malonate (pH 7). The structure was solved by MAD method and refined to an R-factor of 19.5% and R-free of 21.8%. Unlike the two c(7) molecules with known structures, one from G. sulfurreducens (PpcA) and one from Desulfuromonas acetoxidans where all three hemes are bishistidine coordinated, this domain contains a heme which is coordinated by a methionine and a histidine residue. As a result, the corresponding heme could have a higher potential than the other two hemes. The apparent midpoint reduction potential, E(app), of domain C is -105 mV, 50 mV higher than that of PpcA.
+The line below this paragraph, {{ABSTRACT_PUBMED_15133162}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 15133162 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_15133162}}
 ==About this Structure==
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 ==Reference==
 Structure of a novel c7-type three-heme cytochrome domain from a multidomain cytochrome c polymer., Pokkuluri PR, Londer YY, Duke NE, Erickson J, Pessanha M, Salgueiro CA, Schiffer M, Protein Sci. 2004 Jun;13(6):1684-92. Epub 2004 May 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15133162 15133162]
+Family of cytochrome c7-type proteins from Geobacter sulfurreducens: structure of one cytochrome c7 at 1.45 A resolution., Pokkuluri PR, Londer YY, Duke NE, Long WC, Schiffer M, Biochemistry. 2004 Feb 3;43(4):849-59. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14744127 14744127]
 [[Category: Geobacter sulfurreducens]]
 [[Category: Single protein]]
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 [[Category: Heme coordination in c-type cytochrome]]
 [[Category: Multiheme cytochrome c]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:59:29 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 02:31:51 2008''