1ucb: Difference between revisions
New page: left|200px<br /> <applet load="1ucb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ucb, resolution 2.5Å" /> '''STRUCTURE OF UNCOMPL... |
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[[Image:1ucb.gif|left|200px]]<br /> | [[Image:1ucb.gif|left|200px]]<br /><applet load="1ucb" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1ucb, resolution 2.5Å" /> | caption="1ucb, resolution 2.5Å" /> | ||
'''STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)'''<br /> | '''STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)'''<br /> | ||
==Overview== | ==Overview== | ||
The X-ray structure of the uncomplexed human chimeric Fab' of the | The X-ray structure of the uncomplexed human chimeric Fab' of the anti-tumor antibody BR96 has been determined at 2.6 A resolution. The structure has been compared with Lewis Y antigen-complexed structures of BR96 which were determined previously. The comparison reveals segmental motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite its significant main-chain contacts to the carbohydrate antigen. In light of the uncomplexed chimeric Fab' structure reported here, the previously observed high mobility of the CL:CH1 domains of the complexed chimeric BR96 Fab is rationalized as a "swinging" motion approximately about the axis of the elbow bend. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
1UCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1UCB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCB OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:22:54 2008'' | ||
Revision as of 16:23, 21 February 2008
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STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)
OverviewOverview
The X-ray structure of the uncomplexed human chimeric Fab' of the anti-tumor antibody BR96 has been determined at 2.6 A resolution. The structure has been compared with Lewis Y antigen-complexed structures of BR96 which were determined previously. The comparison reveals segmental motions and/or conformational rearrangements of three CDR loops (L1, L3, and H2), whereas CDR H3 does not undergo changes upon complexation despite its significant main-chain contacts to the carbohydrate antigen. In light of the uncomplexed chimeric Fab' structure reported here, the previously observed high mobility of the CL:CH1 domains of the complexed chimeric BR96 Fab is rationalized as a "swinging" motion approximately about the axis of the elbow bend.
DiseaseDisease
Known disease associated with this structure: Kappa light chain deficiency OMIM:[147200]
About this StructureAbout this Structure
1UCB is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
X-ray structure of the uncomplexed anti-tumor antibody BR96 and comparison with its antigen-bound form., Sheriff S, Chang CY, Jeffrey PD, Bajorath J, J Mol Biol. 1996 Jun 28;259(5):938-46. PMID:8683596
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