1rh4: Difference between revisions

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[[Image:1rh4.gif|left|200px]]
[[Image:1rh4.png|left|200px]]


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==Overview==
{{ABSTRACT_9822371}}
Recent advances in computational techniques have allowed the design of precise side-chain packing in proteins with predetermined, naturally occurring backbone structures. Because these methods do not model protein main-chain flexibility, they lack the breadth to explore novel backbone conformations. Here the de novo design of a family of alpha-helical bundle proteins with a right-handed superhelical twist is described. In the design, the overall protein fold was specified by hydrophobic-polar residue patterning, whereas the bundle oligomerization state, detailed main-chain conformation, and interior side-chain rotamers were engineered by computational enumerations of packing in alternate backbone structures. Main-chain flexibility was incorporated through an algebraic parameterization of the backbone. The designed peptides form alpha-helical dimers, trimers, and tetramers in accord with the design goals. The crystal structure of the tetramer matches the designed structure in atomic detail.


==About this Structure==
==About this Structure==
The following page contains interesting information on the relation of 1RH4 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH4 OCA].  
The following page contains interesting information on the relation of 1RH4 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb70_1.html Designer Proteins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH4 OCA].  
==Reference==
High-resolution protein design with backbone freedom., Harbury PB, Plecs JJ, Tidor B, Alber T, Kim PS, Science. 1998 Nov 20;282(5393):1462-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9822371 9822371]
[[Category: Designer Proteins]]
[[Category: Designer Proteins]]
[[Category: Alber, T.]]
[[Category: Alber, T.]]
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[[Category: Coiled coil]]
[[Category: Coiled coil]]
[[Category: De novo design]]
[[Category: De novo design]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 07:29:31 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 17:55:03 2008''

Revision as of 17:55, 26 June 2008

File:1rh4.png

Template:STRUCTURE 1rh4

RH4 DESIGNED RIGHT-HANDED COILED COIL TETRAMER


Template:ABSTRACT 9822371

About this StructureAbout this Structure

The following page contains interesting information on the relation of 1RH4 with [Designer Proteins]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 17:55:03 2008

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