8jz2: Difference between revisions
No edit summary |
No edit summary |
||
| Line 10: | Line 10: | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/A0A861B9Z9_9CYAN A0A861B9Z9_9CYAN] | [https://www.uniprot.org/uniprot/A0A861B9Z9_9CYAN A0A861B9Z9_9CYAN] | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Flavin-dependent halogenases (FDHs) have tremendous applications in synthetic chemistry. A single-component FDH, AetF, exhibits both halogenase and reductase activities in a continuous polypeptide chain. AetF exhibits broad substrate promiscuity and catalyzes the two-step bromination of l-tryptophan (l-Trp) to produce 5-bromotryptophan (5-Br-Trp) and 5,7-dibromo-l-tryptophan (5,7-di-Br-Trp). To elucidate the mechanism of action of AetF, we solved its crystal structure in complex with FAD, FAD/NADP(+), FAD/l-Trp, and FAD/5-Br-Trp at resolutions of 1.92-2.23 A. The obtained crystal structures depict the unprecedented topology of single-component FDH. Structural analysis revealed that the substrate flexibility and dibromination capability of AetF could be attributed to its spacious substrate-binding pocket. In addition, highly-regulated interaction networks between the substrate-recognizing residues and 5-Br-Trp are crucial for the dibromination activity of AetF. Several Ala variants underwent monobromination with >98 % C5-regioselectivity toward l-Trp. These results reveal the catalytic mechanism of single-component FDH for the first time and contribute to efficient FDH protein engineering for biocatalytic halogenation. | |||
Structural and functional insights into the self-sufficient flavin-dependent halogenase.,Dai L, Li H, Dai S, Zhang Q, Zheng H, Hu Y, Guo RT, Chen CC Int J Biol Macromol. 2024 Mar;260(Pt 1):129312. doi: , 10.1016/j.ijbiomac.2024.129312. Epub 2024 Jan 10. PMID:38216020<ref>PMID:38216020</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 8jz2" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Latest revision as of 08:57, 5 June 2024
Crystal structure of AetF in complex with FADCrystal structure of AetF in complex with FAD
Structural highlights
FunctionPublication Abstract from PubMedFlavin-dependent halogenases (FDHs) have tremendous applications in synthetic chemistry. A single-component FDH, AetF, exhibits both halogenase and reductase activities in a continuous polypeptide chain. AetF exhibits broad substrate promiscuity and catalyzes the two-step bromination of l-tryptophan (l-Trp) to produce 5-bromotryptophan (5-Br-Trp) and 5,7-dibromo-l-tryptophan (5,7-di-Br-Trp). To elucidate the mechanism of action of AetF, we solved its crystal structure in complex with FAD, FAD/NADP(+), FAD/l-Trp, and FAD/5-Br-Trp at resolutions of 1.92-2.23 A. The obtained crystal structures depict the unprecedented topology of single-component FDH. Structural analysis revealed that the substrate flexibility and dibromination capability of AetF could be attributed to its spacious substrate-binding pocket. In addition, highly-regulated interaction networks between the substrate-recognizing residues and 5-Br-Trp are crucial for the dibromination activity of AetF. Several Ala variants underwent monobromination with >98 % C5-regioselectivity toward l-Trp. These results reveal the catalytic mechanism of single-component FDH for the first time and contribute to efficient FDH protein engineering for biocatalytic halogenation. Structural and functional insights into the self-sufficient flavin-dependent halogenase.,Dai L, Li H, Dai S, Zhang Q, Zheng H, Hu Y, Guo RT, Chen CC Int J Biol Macromol. 2024 Mar;260(Pt 1):129312. doi: , 10.1016/j.ijbiomac.2024.129312. Epub 2024 Jan 10. PMID:38216020[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||