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| {{STRUCTURE_1osy| PDB=1osy | SCENE= }} | | {{STRUCTURE_1osy| PDB=1osy | SCENE= }} |
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| '''Crystal structure of FIP-Fve fungal immunomodulatory protein'''
| | ===Crystal structure of FIP-Fve fungal immunomodulatory protein=== |
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| ==Overview==
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| Fve, a major fruiting body protein from Flammulina velutipes, a mushroom possessing immunomodulatory activity, stimulates lymphocyte mitogenesis, suppresses systemic anaphylaxis reactions and edema, enhances transcription of IL-2, IFN-gamma and TNF-alpha, and hemagglutinates red blood cells. It appears to be a lectin with specificity for complex cell-surface carbohydrates. Fve is a non-covalently linked homodimer containing no Cys, His or Met residues. It shares sequence similarity only to the other fungal immunomodulatory proteins (FIPs) LZ-8, Gts, Vvo and Vvl, all of unknown structure. The 1.7A structure of Fve solved by single anomalous diffraction of NaBr-soaked crystals is novel: each monomer consists of an N-terminal alpha-helix followed by a fibronectin III (FNIII) fold. The FNIII fold is the first instance of "pseudo-h-type" topology, a transition between the seven beta-stranded s-type and the eight beta-stranded h-type topologies. The structure suggests that dimerization, critical for the activity of FIPs, occurs by 3-D domain swapping of the N-terminal helices and is stabilized predominantly by hydrophobic interactions. The structure of Fve is the first in this lectin family to be reported, and the first of an FNIII domain-containing protein of fungal origin.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12948495}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12948495 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12948495}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Immunomodulatory]] | | [[Category: Immunomodulatory]] |
| [[Category: Lectin]] | | [[Category: Lectin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:14:39 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:25:06 2008'' |