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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n3r OCA], [https://pdbe.org/2n3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n3r RCSB], [https://www.ebi.ac.uk/pdbsum/2n3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n3r ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n3r OCA], [https://pdbe.org/2n3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n3r RCSB], [https://www.ebi.ac.uk/pdbsum/2n3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n3r ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| As part of an effort to structurally characterize the complete Neurospora VS ribozyme, NMR solution structures of several subdomains have been previously determined, including the internal loops of domains I and VI, the I/V kissing-loop interaction and the III-IV-V junction. Here, we expand this work by determining the NMR structure of a 62-nucleotide RNA (J236) that encompasses the VS ribozyme II-III-VI three-way junction and its adjoining stems. In addition, we localize Mg2+-binding sites within this structure using Mn2+-induced paramagnetic relaxation enhancement. The NMR structure of the J236 RNA displays a family C topology with a compact core stabilized by continuous stacking of stems II and III, a cis WC/WC G*A base pair, two base triples and two Mg2+ ions. Moreover, it reveals a remote tertiary interaction between the adenine bulges of stems II and VI. Additional NMR studies demonstrate that both this bulge-bulge interaction and Mg2+ ions are critical for the stable folding of the II-III-VI junction. The NMR structure of the J236 RNA is consistent with biochemical studies on the complete VS ribozyme, but not with biophysical studies performed with a minimal II-III-VI junction that does not contain the II-VI bulge-bulge interaction. Together with previous NMR studies, our findings provide important new insights into the three-dimensional architecture of this unique ribozyme.
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| The NMR structure of the II-III-VI three-way junction from the Neurospora VS ribozyme reveals a critical tertiary interaction and provides new insights into the global ribozyme structure.,Bonneau E, Girard N, Lemieux S, Legault P RNA. 2015 Jun 29. PMID:26124200<ref>PMID:26124200</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2n3r" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Ribozyme 3D structures|Ribozyme 3D structures]] | | *[[Ribozyme 3D structures|Ribozyme 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |