1ouk: Difference between revisions
New page: left|200px<br /> <applet load="1ouk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ouk, resolution 2.5Å" /> '''The structure of p38... |
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'''The structure of p38 alpha in complex with a pyridinylimidazole inhibitor'''<br /> | '''The structure of p38 alpha in complex with a pyridinylimidazole inhibitor'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1OUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and 084 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http:// | 1OUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=084:'>084</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUK OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: map kinase]] | [[Category: map kinase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:36:09 2008'' | ||
Revision as of 17:36, 15 February 2008
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The structure of p38 alpha in complex with a pyridinylimidazole inhibitor
OverviewOverview
The quinazolinone and pyridol-pyrimidine classes of p38 MAP kinase, inhibitors have a previously unseen degree of specificity for p38 over, other MAP kinases. Comparison of the crystal structures of p38 bound to, four different compounds shows that binding of the more specific molecules, is characterized by a peptide flip between Met109 and Gly110. Gly110 is a, residue specific to the alpha, beta and gamma isoforms of p38. The delta, isoform and the other MAP kinases have bulkier residues in this position., These residues would likely make the peptide flip energetically, unfavorable, thus explaining the selectivity of binding. To test this, hypothesis, we constructed G110A and G110D mutants of p38 and measured the, potency of several compounds against them. The results confirm that the, selectivity of quinazolinones and pyridol-pyrimidines results from the, presence of a glycine in position 110. This unique mode of binding may be, exploited in the design of new p38 inhibitors.
About this StructureAbout this Structure
1OUK is a Single protein structure of sequence from Homo sapiens with and as ligands. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for p38alpha MAP kinase quinazolinone and pyridol-pyrimidine inhibitor specificity., Fitzgerald CE, Patel SB, Becker JW, Cameron PM, Zaller D, Pikounis VB, O'Keefe SJ, Scapin G, Nat Struct Biol. 2003 Sep;10(9):764-9. Epub 2003 Aug 3. PMID:12897767
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