1n2f: Difference between revisions

Revision as of 06:37, 28 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1n2f.png

Template:STRUCTURE 1n2f

CRYSTAL STRUCTURE OF P. AERUGINOSA OHRCRYSTAL STRUCTURE OF P. AERUGINOSA OHR

Template:ABSTRACT PUBMED 12485986

About this StructureAbout this Structure

1N2F is a Single protein structure of sequence from Pseudomonas aeruginosa pao1. Full crystallographic information is available from OCA.

ReferenceReference

Structural and functional characterization of the Pseudomonas hydroperoxide resistance protein Ohr., Lesniak J, Barton WA, Nikolov DB, EMBO J. 2002 Dec 16;21(24):6649-59. PMID:12485986

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-'''CRYSTAL STRUCTURE OF P. AERUGINOSA OHR'''
+===CRYSTAL STRUCTURE OF P. AERUGINOSA OHR===
-==Overview==
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-Bacteria have developed complex strategies to detoxify and repair damage caused by reactive oxygen species. These compounds, produced during bacterial aerobic respiration as well as by the host immune system cells as a defense mechanism against the pathogenic microorganisms, have the ability to damage nucleic acids, proteins and phospholipid membranes. Here we describe the crystal structure of Pseudomonas aeruginosa Ohr, a member of a recently discovered family of organic hydroperoxide resistance proteins. Ohr is a tightly folded homodimer, with a novel alpha/beta fold, and contains two active sites located at the monomer interface on opposite sides of the molecule. Using in vitro assays, we demonstrate that Ohr functions directly as a hydroperoxide reductase, converting both inorganic and organic hydroperoxides to less toxic metabolites. Site-directed mutagenesis confirms that the two conserved cysteines in each active site are essential for catalytic activity. We propose that the Ohr catalytic mechanism is similar to that of the structurally unrelated peroxiredoxins, directly utilizing highly reactive cysteine thiol groups to elicit hydroperoxide reduction.
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 [[Category: Nikolov, D B.]]
 [[Category: Peroxide reductase]]
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