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| {{STRUCTURE_1m4z| PDB=1m4z | SCENE= }} | | {{STRUCTURE_1m4z| PDB=1m4z | SCENE= }} |
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| '''Crystal structure of the N-terminal BAH domain of Orc1p'''
| | ===Crystal structure of the N-terminal BAH domain of Orc1p=== |
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| ==Overview==
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| The N-terminal domain of the largest subunit of the Saccharomyces cerevisiae origin recognition complex (Orc1p) functions in transcriptional silencing and contains a bromo-adjacent homology (BAH) domain found in some chromatin-associated proteins including Sir3p. The 2.2 A crystal structure of the N-terminal domain of Orc1p revealed a BAH core and a non-conserved helical sub-domain. Mutational analyses demonstrated that the helical sub-domain was necessary and sufficient to bind Sir1p, and critical for targeting Sir1p primarily to the cis-acting E silencers at the HMR and HML silent chromatin domains. In the absence of the BAH domain, approximately 14-20% of cells in a population were silenced at the HML locus. Moreover, the distributions of the Sir2p, Sir3p and Sir4p proteins, while normal, were at levels lower than found in wild-type cells. Thus, in the absence of the Orc1p BAH domain, HML resembled silencing of genes adjacent to telomeres. These data are consistent with the view that the Orc1p-Sir1p interaction at the E silencers ensures stable inheritance of pre-established Sir2p, Sir3p and Sir4p complexes at the silent mating type loci.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12198162}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12198162 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12198162}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Dna replication]] | | [[Category: Dna replication]] |
| [[Category: Transcriptional silencing]] | | [[Category: Transcriptional silencing]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:38:14 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 23:13:16 2008'' |