1lv2: Difference between revisions
New page: left|200px<br /> <applet load="1lv2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lv2, resolution 2.7Å" /> '''Hepatocyte Nuclear F... |
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[[Image:1lv2.gif|left|200px]]<br /> | [[Image:1lv2.gif|left|200px]]<br /><applet load="1lv2" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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caption="1lv2, resolution 2.7Å" /> | caption="1lv2, resolution 2.7Å" /> | ||
'''Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids'''<br /> | '''Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1LV2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with PLM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1LV2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PLM:'>PLM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LV2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:20:37 2008'' | ||
Revision as of 17:20, 15 February 2008
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Hepatocyte Nuclear Factor 4 is a Transcription Factor that Constitutively Binds Fatty Acids
OverviewOverview
The 2.7 A X-ray crystal structure of the HNF4gamma ligand binding domain, (LBD) revealed the presence of a fatty acid within the pocket, with the, AF2 helix in a conformation characteristic of a transcriptionally active, nuclear receptor. GC/MS and NMR analysis of chloroform/methanol extracts, from purified HNF4alpha and HNF4gamma LBDs identified mixtures of, saturated and cis-monounsaturated C14-18 fatty acids. The purified HNF4, LBDs interacted with nuclear receptor coactivators, and both HNF4 subtypes, show high constitutive activity in transient transfection assays, which, was reduced by mutations designed to interfere with fatty acid binding., The endogenous fatty acids did not readily exchange with radiolabeled, palmitic acid, and all attempts to displace them without denaturing the, protein failed. Our results suggest that the HNF4s may be transcription, factors that are constitutively bound to fatty acids.
About this StructureAbout this Structure
1LV2 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Hepatocyte nuclear factor 4 is a transcription factor that constitutively binds fatty acids., Wisely GB, Miller AB, Davis RG, Thornquest AD Jr, Johnson R, Spitzer T, Sefler A, Shearer B, Moore JT, Miller AB, Willson TM, Williams SP, Structure. 2002 Sep;10(9):1225-34. PMID:12220494
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