User:Daniel Key Takemoto/Sandbox 1: Difference between revisions

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==RGG motif==

An important motif of the FMRP is the <scene name='96/969643/Rgg_box/1'>RGG box</scene>, which the protein uses to bind to guanine G-quadruplexes, a structure that consists of nucleic acid folding in which four guanines arrange in a planar conformation stabilized by Hoogsteen-~~trype~~ hydrogen bonds, named tetrad, and they are stabilized by a K+ cation (green ball in the image). The RNA being represented is the ''sc1'' RNA, and the interaction between them was visualized through NMR. There are <scene name='96/969643/Sc1_gquadruplexes/1'>three G-Quadruplexes</scene> in the ''sc1'' RNA. FMRP RGG motifs seem to prefer binding to specific structures.

An important motif of the FMRP is the <scene name='96/969643/Rgg_box/1'>RGG box</scene>, which the protein uses to bind to guanine G-quadruplexes, a structure that consists of nucleic acid folding in which four guanines arrange in a planar conformation stabilized by Hoogsteen-type hydrogen bonds, named tetrad, and they are stabilized by a K+ cation (green ball in the image). The RNA being represented is the ''sc1'' RNA, and the interaction between them was visualized through NMR. There are <scene name='96/969643/Sc1_gquadruplexes/1'>three G-Quadruplexes</scene> in the ''sc1'' RNA. FMRP RGG motifs seem to prefer binding to specific structures.

Different domains and motifs mediate the RNA binding mechanism, and the exon 15-encoded <scene name='96/969643/Rgg_motif/2'>RGG motif</scene> (arginine - glycine - glycine) and the binding of FMRP to G-rich RNAs in vitro requires only the RGG motif, which occurs through the binding of a '''hydrogen bond between an RNA base and a aminoacid residue'''. The FMRP RGG motif is located in the C-terminal region of the protein and is well conserved in vertebrates. Crystal structure of the complex between the human FMRP RGG motif and G-quadruplex RNA The RGG motif binds to G-quadruplexes when it adopts a sharp turn and specifically binds to guanines from two consecutive G-C base pairs in the duplex-quadruplex junction. Several tetrads can stack in a single G-quadruplex structure and be stabilized further by potassium cations, in the case of FMRP targets, whereas they are destabilized by lithium cations.

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 ==RGG motif==
-An important motif of the FMRP is the <scene name='96/969643/Rgg_box/1'>RGG box</scene>, which the protein uses to bind to guanine G-quadruplexes, a structure that consists of nucleic acid folding in which four guanines arrange in a planar conformation stabilized by Hoogsteen-trype hydrogen bonds, named tetrad, and they are stabilized by a K+ cation (green ball in the image). The RNA being represented is the ''sc1'' RNA, and the interaction between them was visualized through NMR. There are <scene name='96/969643/Sc1_gquadruplexes/1'>three G-Quadruplexes</scene> in the ''sc1'' RNA. FMRP RGG motifs seem to prefer binding to specific structures.
+An important motif of the FMRP is the <scene name='96/969643/Rgg_box/1'>RGG box</scene>, which the protein uses to bind to guanine G-quadruplexes, a structure that consists of nucleic acid folding in which four guanines arrange in a planar conformation stabilized by Hoogsteen-type hydrogen bonds, named tetrad, and they are stabilized by a K+ cation (green ball in the image). The RNA being represented is the ''sc1'' RNA, and the interaction between them was visualized through NMR. There are <scene name='96/969643/Sc1_gquadruplexes/1'>three G-Quadruplexes</scene> in the ''sc1'' RNA. FMRP RGG motifs seem to prefer binding to specific structures.
 Different domains and motifs mediate the RNA binding mechanism, and the exon 15-encoded <scene name='96/969643/Rgg_motif/2'>RGG motif</scene> (arginine - glycine - glycine) and the binding of FMRP to G-rich RNAs in vitro requires only the RGG motif, which occurs through the binding of a '''hydrogen bond between an RNA base and a aminoacid residue'''. The FMRP RGG motif is located in the C-terminal region of the protein and is well conserved in vertebrates. Crystal structure of the complex between the human FMRP RGG motif and G-quadruplex RNA The RGG motif binds to G-quadruplexes when it adopts a sharp turn and specifically binds to guanines from two consecutive G-C base pairs in the duplex-quadruplex junction. Several tetrads can stack in a single G-quadruplex structure and be stabilized further by potassium cations, in the case of FMRP targets, whereas they are destabilized by lithium cations.