1kpc: Difference between revisions
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[[Image:1kpc.gif|left|200px]]<br /> | [[Image:1kpc.gif|left|200px]]<br /><applet load="1kpc" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1kpc" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="1kpc, resolution 2.2Å" /> | caption="1kpc, resolution 2.2Å" /> | ||
'''PKCI-1-APO+ZINC'''<br /> | '''PKCI-1-APO+ZINC'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1KPC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. | 1KPC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Known structural/functional Sites: <scene name='pdbsite=ZNA:Triad Of Histidines Potentially Involved In Zn Coordination'>ZNA</scene>, <scene name='pdbsite=ZNB:Triad Of Histidines Potentially Involved In Zn Coordination'>ZNB</scene>, <scene name='pdbsite=ZNC:Triad Of Histidines Potentially Involved In Zn Coordination'>ZNC</scene> and <scene name='pdbsite=ZND:Triad Of Histidines Potentially Involved In Zn Coordination'>ZND</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1KPC OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: zinc binding protein]] | [[Category: zinc binding protein]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 16:41:38 2007'' | ||
Revision as of 17:31, 18 December 2007
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PKCI-1-APO+ZINC
OverviewOverview
The three-dimensional structure of protein kinase C interacting protein 1, (PKCI-1) has been solved to high resolution by x-ray crystallography using, single isomorphous replacement with anomalous scattering. The gene, encoding human PKCI-1 was cloned from a cDNA library by using a partial, sequence obtained from interactions identified in the yeast two-hybrid, system between PKCI-1 and the regulatory domain of protein kinase C-beta., The PKCI-1 protein was expressed in Pichia pastoris as a dimer of two, 13.7-kDa polypeptides. PKCI-1 is a member of the HIT family of proteins, shown by sequence identity to be conserved in a broad range of organisms, including mycoplasma, plants, and humans. Despite the ubiquity of this, protein sequence in nature, no distinct function has been shown for the, protein product in vitro or in vivo. The PKCI-1 protomer has an alpha+beta, meander fold containing a five-stranded antiparallel sheet and two, helices. Two protomers come together to form a 10-stranded antiparallel, sheet with extensive contacts between a helix and carboxy terminal amino, acids of a protomer with the corresponding amino acids in the other, protomer. PKCI-1 has been shown to interact specifically with zinc. The, three-dimensional structure has been solved in the presence and absence of, zinc and in two crystal forms. The structure of human PKCI-1 provides a, model of this family of proteins which suggests a stable fold conserved, throughout nature.
About this StructureAbout this Structure
1KPC is a Single protein structure of sequence from Homo sapiens. Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
ReferenceReference
Three-dimensional structure of human protein kinase C interacting protein 1, a member of the HIT family of proteins., Lima CD, Klein MG, Weinstein IB, Hendrickson WA, Proc Natl Acad Sci U S A. 1996 May 28;93(11):5357-62. PMID:8643579
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