1l5e: Difference between revisions

Revision as of 11:44, 2 July 2008

This article has been automatically seeded. Changes to this page should pertain to the PDB entry only and not to the protein or biomolecule in general.

File:1l5e.png

Template:STRUCTURE 1l5e

The domain-swapped dimer of CV-N in solutionThe domain-swapped dimer of CV-N in solution

Template:ABSTRACT PUBMED 12015150

About this StructureAbout this Structure

1L5E is a Single protein structure of sequence from Nostoc ellipsosporum. Full experimental information is available from OCA.

ReferenceReference

The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures., Barrientos LG, Louis JM, Botos I, Mori T, Han Z, O'Keefe BR, Boyd MR, Wlodawer A, Gronenborn AM, Structure. 2002 May;10(5):673-86. PMID:12015150

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 {{STRUCTURE_1l5e|  PDB=1l5e  |  SCENE=  }}
-'''The domain-swapped dimer of CV-N in solution'''
+===The domain-swapped dimer of CV-N in solution===
-==Overview==
+<!--
-The structure of the potent HIV-inactivating protein cyanovirin-N was previously found by NMR to be a monomer in solution and a domain-swapped dimer by X-ray crystallography. Here we demonstrate that, in solution, CV-N can exist both in monomeric and in domain-swapped dimeric form. The dimer is a metastable, kinetically trapped structure at neutral pH and room temperature. Based on orientational NMR constraints, we show that the domain-swapped solution dimer is similar to structures in two different crystal forms, exhibiting solely a small reorientation around the hinge region. Mutation of the single proline residue in the hinge to glycine significantly stabilizes the protein in both its monomeric and dimeric forms. By contrast, mutation of the neighboring serine to proline results in an exclusively dimeric protein, caused by a drastic destabilization of the monomer.
+The line below this paragraph, {{ABSTRACT_PUBMED_12015150}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12015150 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12015150}}
 ==About this Structure==
-L5E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5E OCA].
+L5E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5E OCA].
 ==Reference==
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 [[Category: Protein folding]]
 [[Category: X-ray]]
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