1kvy: Difference between revisions

Revision as of 11:07, 2 July 2008

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File:1kvy.png

Template:STRUCTURE 1kvy

CARBOXYLIC ESTER HYDROLASE, SINGLE MUTANT D49E COORDINATED TO CALCIUMCARBOXYLIC ESTER HYDROLASE, SINGLE MUTANT D49E COORDINATED TO CALCIUM

Template:ABSTRACT PUBMED 10089353

About this StructureAbout this Structure

1KVY is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

Structures of the catalytic site mutants D99A and H48Q and the calcium-loop mutant D49E of phospholipase A2., Sekar K, Biswas R, Li Y, Tsai M, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):443-7. PMID:10089353

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-'''CARBOXYLIC ESTER HYDROLASE, SINGLE MUTANT D49E COORDINATED TO CALCIUM'''
+===CARBOXYLIC ESTER HYDROLASE, SINGLE MUTANT D49E COORDINATED TO CALCIUM===
-==Overview==
+<!--
-Crystal structures of the active-site mutants D99A and H48Q and the calcium-loop mutant D49E of bovine phospholipase A2 have been determined at around 1.9 A resolution. The D99A mutant is isomorphous to the orthorhombic recombinant enzyme, space group P212121. The H48Q and the calcium-loop mutant D49E are isomorphous to the trigonal recombinant enzyme, space group P3121. The two active-site mutants show no major structural perturbations. The structural water is absent in D99A and, therefore, the hydrogen-bonding scheme is changed. In H48Q, the catalytic water is present and hydrogen bonded to Gln48 N, but the second water found in native His48 is absent. In the calcium-loop mutant D49E, the two water molecules forming the pentagonal bipyramid around calcium are absent and only one O atom of the Glu49 carboxylate group is coordinated to calcium, resulting in only four ligands.
+The line below this paragraph, {{ABSTRACT_PUBMED_10089353}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 10089353 is the PubMed ID number.
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+{{ABSTRACT_PUBMED_10089353}}
 ==About this Structure==
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 [[Category: Hydrolase]]
 [[Category: Mutant]]
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