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| [[Image:1ko3.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1ko3| PDB=1ko3 | SCENE= }} | | {{STRUCTURE_1ko3| PDB=1ko3 | SCENE= }} |
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| '''VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa with Cys221 reduced'''
| | ===VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa with Cys221 reduced=== |
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| ==Overview==
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| The crystal structures of the universally widespread metallo-beta-lactamase (MBL) Verona integron-encoded MBL (VIM)-2 from Pseudomonas aeruginosa have been solved in their native form as well as in an unexpected oxidised form. This carbapenem-hydrolysing enzyme belongs to the so-called B1 subfamily of MBLs and shares the folding of alpha beta/beta alpha sandwich, consisting of a core of beta-sheet surrounded by alpha-helices. Surprisingly, it showed a high tendency to be strongly oxidised at the catalytic cysteine located in the Cys site, Cys221, which, in the oxidised structure, becomes a cysteinesulfonic residue. Its native structure was obtained only in the presence of Tris(2-carboxyethyl)phosphine. This oxidation might be a consequence of a lower affinity for the second Zn located in the Cys site that would also explain the observed susceptibility of VIM-2 to chelating agents. This modification, if present in nature, might play a role in catalytic down-regulation. Comparison between native and oxidised VIM-2 and a predicted model of VIM-1 (which shows one residue different in the Cys site compared with VIM-2) is performed to explain the different activities and antibiotic specificities. | | The line below this paragraph, {{ABSTRACT_PUBMED_18061205}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18061205 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18061205}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Alpha-beta/beta-alpha fold]] | | [[Category: Alpha-beta/beta-alpha fold]] |
| [[Category: Hydrolase]] | | [[Category: Hydrolase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:58:13 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:37:35 2008'' |