Sandbox Reserved 1789: Difference between revisions
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=== PP1C === | === PP1C === | ||
<scene name='95/952717/Pp1c/ | <scene name='95/952717/Pp1c/2'>PP1C</scene> is a phosphatase. After forming a ternary complex, the hydrophobic active site on PP1C interacts with Raf and dephosphorylate Ser 259. PP1C's active site is adjacent to a hydrophobic binding pocket that binds to the C-terminal phosphoserine, located on the N-terminus of RAF, the target for dephosphorylation. PP1C can act as a phosphatase in the absence of SHOC2 but PP1C lacks intrinsic substrate selectively. The SMP complex formation endows PP1C with specificity for RAF <ref name="Hauseman" />. | ||
The mechanism that PP1C uses to catalyze the dephosphorylation is mainly through donating a hydrogen atom to a phosphate group on the C-terminal of a [https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/phosphoserine. phosphoserine] on Raf <ref name="Kubicek"/>. This makes the phosphate group a good leaving group and it breaks off <ref name="Kubicek"/>. This catalysis is done by the serine-threonine alpha catalytic site on PP1C <ref name="Kubicek"/>. In this catalytic site, there are two Manganese ions and one calcium ion <ref name="Kubicek"/>. These metal ions are necessary in stablizing this catalytic site and there are a lot of polar negative residues in this region <ref name="Kubicek"/> | The mechanism that PP1C uses to catalyze the dephosphorylation is mainly through donating a hydrogen atom to a phosphate group on the C-terminal of a [https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/phosphoserine. phosphoserine] on Raf <ref name="Kubicek"/>. This makes the phosphate group a good leaving group and it breaks off <ref name="Kubicek"/>. This catalysis is done by the serine-threonine alpha catalytic site on PP1C <ref name="Kubicek"/>. In this catalytic site, there are two Manganese ions and one calcium ion <ref name="Kubicek"/>. These metal ions are necessary in stablizing this catalytic site and there are a lot of polar negative residues in this region <ref name="Kubicek"/> | ||