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| [[Image:1kio.jpg|left|200px]] | | {{Seed}} |
| | [[Image:1kio.png|left|200px]] |
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| {{STRUCTURE_1kio| PDB=1kio | SCENE= }} | | {{STRUCTURE_1kio| PDB=1kio | SCENE= }} |
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| '''SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]'''
| | ===SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]=== |
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| ==Overview==
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| The solution structure of three small serine proteinase inhibitors, two natural and one engineered protein, SGCI (Schistocerca gregaria chymotrypsin inhibitor), SGCI[L30R, K31M] and SGTI (Schistocerca gregaria trypsin inhibitor), were determined by homonuclear NMR-spectroscopy. The molecules exhibit different specificities towards target proteinases, where SGCI is a good chymotrypsin inhibitor, its mutant is a potent trypsin inhibitor, and SGTI inhibits both proteinases weakly. Interestingly, SGTI is a much better inhibitor of insect proteinases than of the mammalian ones used in common assays. All three molecules have a similar fold composed from three antiparallel beta-pleated sheets with three disulfide bridges. The proteinase binding loop has a somewhat distinct geometry in all three peptides. Moreover, the stabilization of the structure is different in SGCI and SGTI. Proton-deuterium exchange experiments are indicative of a highly rigid core in SGTI but not in SGCI. We suggest that the observed structural properties play a significant role in the specificity of these inhibitors. | | The line below this paragraph, {{ABSTRACT_PUBMED_11856311}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11856311 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11856311}} |
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| ==About this Structure== | | ==About this Structure== |
| 1KIO is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIO OCA]. | | 1KIO is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIO OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Protease inhibitor]] | | [[Category: Protease inhibitor]] |
| [[Category: Specificity]] | | [[Category: Specificity]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:47:38 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:24:09 2008'' |