1kaf: Difference between revisions

Revision as of 10:02, 2 July 2008

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File:1kaf.png

Template:STRUCTURE 1kaf

DNA Binding Domain Of The Phage T4 Transcription Factor MotA (AA105-211)DNA Binding Domain Of The Phage T4 Transcription Factor MotA (AA105-211)

Template:ABSTRACT PUBMED 11918797

About this StructureAbout this Structure

1KAF is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

ReferenceReference

The MotA transcription factor from bacteriophage T4 contains a novel DNA-binding domain: the 'double wing' motif., Li N, Sickmier EA, Zhang R, Joachimiak A, White SW, Mol Microbiol. 2002 Mar;43(5):1079-88. PMID:11918797

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''DNA Binding Domain Of The Phage T4 Transcription Factor MotA (AA105-211)'''
+===DNA Binding Domain Of The Phage T4 Transcription Factor MotA (AA105-211)===
-==Overview==
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-MotA is a transcription factor from bacteriophage T4 that helps adapt the host Escherichia coli transcription apparatus to T4 middle promoters. We have determined the crystal structure of the C-terminal DNA-binding domain of MotA (MotCF) to 1.6 A resolution using multiwavelength, anomalous diffraction methods. The structure reveals a novel DNA-binding alpha/beta motif that contains an exposed beta-sheet surface that mediates interactions with the DNA. Independent biochemical experiments have shown that MotCF binds to one surface of a single turn of DNA through interactions in adjacent major and minor grooves. We present a model of the interaction in which beta-ribbons at opposite corners of the six-stranded beta-sheet penetrate the DNA grooves, and call the motif a 'double wing' to emphasize similarities to the 'winged-helix' motif. The model is consistent with data on how MotA functions at middle promoters, and provides an explanation for why MotA can form non-specific multimers on DNA.
+The line below this paragraph, {{ABSTRACT_PUBMED_11918797}}, adds the Publication Abstract to the page
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 [[Category: Structural genomic]]
 [[Category: X-ray crystallography]]
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