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| [[Image:1k61.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1k61| PDB=1k61 | SCENE= }} | | {{STRUCTURE_1k61| PDB=1k61 | SCENE= }} |
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| '''MATALPHA2 HOMEODOMAIN BOUND TO DNA'''
| | ===MATALPHA2 HOMEODOMAIN BOUND TO DNA=== |
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| ==Overview==
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| Hoogsteen base pairs within duplex DNA typically are only observed in regions containing significant distortion or near sites of drug intercalation. We report here the observation of a Hoogsteen base pair embedded within undistorted, unmodified B-DNA. The Hoogsteen base pair, consisting of a syn adenine base paired with an anti thymine base, is found in the 2.1 A resolution structure of the MATalpha2 homeodomain bound to DNA in a region where a specifically and a non-specifically bound homeodomain contact overlapping sites. NMR studies of the free DNA show no evidence of Hoogsteen base pair formation, suggesting that protein binding favors the transition from a Watson-Crick to a Hoogsteen base pair. Molecular dynamics simulations of the homeodomain-DNA complex support a role for the non-specifically bound protein in favoring Hoogsteen base pair formation. The presence of a Hoogsteen base pair in the crystal structure of a protein-DNA complex raises the possibility that Hoogsteen base pairs could occur within duplex DNA and play a hitherto unrecognized role in transcription, replication and other cellular processes.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12466549}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12466549 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12466549}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Hoogsteen base pair]] | | [[Category: Hoogsteen base pair]] |
| [[Category: Protein-dna complex]] | | [[Category: Protein-dna complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:21:05 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 09:50:18 2008'' |