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| [[Image:1jmc.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1jmc| PDB=1jmc | SCENE= }} | | {{STRUCTURE_1jmc| PDB=1jmc | SCENE= }} |
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| '''SINGLE STRANDED DNA-BINDING DOMAIN OF HUMAN REPLICATION PROTEIN A BOUND TO SINGLE STRANDED DNA, RPA70 SUBUNIT, RESIDUES 183-420'''
| | ===SINGLE STRANDED DNA-BINDING DOMAIN OF HUMAN REPLICATION PROTEIN A BOUND TO SINGLE STRANDED DNA, RPA70 SUBUNIT, RESIDUES 183-420=== |
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| ==Overview==
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| The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. The structures of four SSBs have been solved, but the molecular details of the interaction of SSBs with DNA remain speculative. We report here the crystal structure at 2.4 A resolution of the single-stranded-DNA-binding domain of human replication protein A (RPA) bound to DNA. Replication protein A is a heterotrimeric SSB that is highly conserved in eukaryotes. The largest subunit, RPA70, binds to single-stranded (ss)DNA and mediates interactions with many cellular and viral proteins. The DNA-binding domain, which lies in the middle of RPA70, comprises two structurally homologous subdomains oriented in tandem. The ssDNA lies in a channel that extends from one subdomain to the other. The structure of each RPA70 subdomain is similar to those of the bacteriophage SSBs, indicating that the mechanism of ssDNA-binding is conserved. | | The line below this paragraph, {{ABSTRACT_PUBMED_8990123}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 8990123 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_8990123}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Protein-ssdna complex]] | | [[Category: Protein-ssdna complex]] |
| [[Category: Single stranded dna-binding protein]] | | [[Category: Single stranded dna-binding protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:24:31 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:27:52 2008'' |