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| {{STRUCTURE_1jl2| PDB=1jl2 | SCENE= }} | | {{STRUCTURE_1jl2| PDB=1jl2 | SCENE= }} |
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| '''Crystal structure of TCEO RNase H-a chimera combining the folding core from T. thermophilus RNase H and the remaining region of E. coli RNase H'''
| | ===Crystal structure of TCEO RNase H-a chimera combining the folding core from T. thermophilus RNase H and the remaining region of E. coli RNase H=== |
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| ==Overview==
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| To investigate the contribution of the folding cores to the thermodynamic stability of RNases H, we used rational design to create two chimeras composed of parts of a thermophilic and a mesophilic RNase H. Each chimera combines the folding core from one parent protein and the remaining parts of the other. Both chimeras form active, well-folded RNases H. Stability curves, based on CD-monitored chemical denaturations, show that the chimera with the thermophilic core is more stable, has a higher midpoint of thermal denaturation, and a lower change in heat capacity (DeltaCp) upon unfolding than the chimera with the mesophilic core. A possible explanation for the low DeltaCp of both the parent thermophilic RNase H and the chimera with the thermophilic core is the residual structure in the denatured state. On the basis of the studied parameters, the chimera with the thermophilic core resembles a true thermophilic protein. Our results suggest that the folding core plays an essential role in conferring thermodynamic parameters to RNases H.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11790848}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11790848 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11790848}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Robic, S.]] | | [[Category: Robic, S.]] |
| [[Category: Mixed alpha-beta protein]] | | [[Category: Mixed alpha-beta protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:21:20 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:24:43 2008'' |