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| [[Image:1jfx.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1jfx| PDB=1jfx | SCENE= }} | | {{STRUCTURE_1jfx| PDB=1jfx | SCENE= }} |
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| '''Crystal structure of the bacterial lysozyme from Streptomyces coelicolor at 1.65 A resolution'''
| | ===Crystal structure of the bacterial lysozyme from Streptomyces coelicolor at 1.65 A resolution=== |
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| ==Overview==
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| Cellosyl is a bacterial muramidase from Streptomyces coelicolor. Similar to other lysozymes, the enzyme cleaves the beta-1,4-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine units, but it also exhibits a beta-1,4-N,6-O-diacetylmuramidase activity. The latter enables Cellosyl to degrade the cell walls of Staphylococcus aureus, which are not hydrolyzed by chicken-, goose-, or bacteriophage T4-type lysozymes. The enzymatic activity and amino acid sequence of Cellosyl group it with lysozymes of the Chalaropsis type, for which no detailed structural information has been available so far. The crystal structure of Cellosyl from S. coelicolor has been determined to a resolution of 1.65 A and refined to an R-factor of 15.2%. The enzyme is comprised of a single domain and possesses an unusual beta/alpha-barrel fold. The last strand, beta 8, of the (beta/alpha)(5)beta(3)-barrel is found to be antiparallel to strands beta 7 and beta 1. Asp-9, Asp-98, and Glu-100 are located at the active site. The structure of Cellosyl exhibits a new lysozyme fold and represents a new class of polysaccharide-hydrolyzing beta/alpha-barrels.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11427528}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11427528 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11427528}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Lysozyme]] | | [[Category: Lysozyme]] |
| [[Category: N-acetylmuramidase]] | | [[Category: N-acetylmuramidase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:10:34 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:11:22 2008'' |