6z6d: Difference between revisions

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<StructureSection load='6z6d' size='340' side='right'caption='[[6z6d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='6z6d' size='340' side='right'caption='[[6z6d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6z6d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_HK97 Escherichia virus HK97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z6D FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z6D FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z6d OCA], [https://pdbe.org/6z6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z6d RCSB], [https://www.ebi.ac.uk/pdbsum/6z6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z6d ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z6d OCA], [https://pdbe.org/6z6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z6d RCSB], [https://www.ebi.ac.uk/pdbsum/6z6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z6d ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9MCT1_BPHK7 Q9MCT1_BPHK7]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many essential cellular processes rely on substrate rotation or translocation by a multi-subunit, ring-type NTPase. A large number of double-stranded DNA viruses, including tailed bacteriophages and herpes viruses, use a homomeric ring ATPase to processively translocate viral genomic DNA into procapsids during assembly. Our current understanding of viral DNA packaging comes from three archetypal bacteriophage systems: cos, pac and phi29. Detailed mechanistic understanding exists for pac and phi29, but not for cos. Here, we reconstituted in vitro a cos packaging system based on bacteriophage HK97 and provided a detailed biochemical and structural description. We used a photobleaching-based, single-molecule assay to determine the stoichiometry of the DNA-translocating ATPase large terminase. Crystal structures of the large terminase and DNA-recruiting small terminase, a first for a biochemically defined cos system, reveal mechanistic similarities between cos and pac systems. At the same time, mutational and biochemical analyses indicate a new regulatory mechanism for ATPase multimerization and coordination in the HK97 system. This work therefore establishes a framework for studying the evolutionary relationships between ATP-dependent DNA translocation machineries in double-stranded DNA viruses.
Structural basis of DNA packaging by a ring-type ATPase from an archetypal viral system.,Fung HKH, Grimes S, Huet A, Duda RL, Chechik M, Gault J, Robinson CV, Hendrix RW, Jardine PJ, Conway JF, Baumann CG, Antson AA Nucleic Acids Res. 2022 Aug 26;50(15):8719-8732. doi: 10.1093/nar/gkac647. PMID:35947691<ref>PMID:35947691</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6z6d" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Terminase 3D Structures|Terminase 3D Structures]]
*[[Terminase 3D Structures|Terminase 3D Structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia virus HK97]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Antson AA]]
[[Category: Antson AA]]

Latest revision as of 09:10, 19 June 2024

Crystal structure of the HK97 bacteriophage large terminaseCrystal structure of the HK97 bacteriophage large terminase

Structural highlights

Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

6z6d, resolution 2.20Å

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