1dwh: Difference between revisions
New page: left|200px<br /> <applet load="1dwh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dwh, resolution 2.0Å" /> '''STUDY ON RADIATION D... |
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==About this Structure== | ==About this Structure== | ||
1DWH is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba]] with NAG, ZN, SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.3.1 3.2.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DWH OCA]]. | 1DWH is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Sinapis_alba Sinapis alba]] with NAG, ZN, SO4 and GOL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_3.2.1.147 Transferred entry: 3.2.1.147]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.3.1 3.2.3.1]]. Structure known Active Site: ZNB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DWH OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Sinapis alba]] | [[Category: Sinapis alba]] | ||
[[Category: Transferred entry: 3.2.1.147]] | |||
[[Category: Burmeister, W.P.]] | [[Category: Burmeister, W.P.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: radiolysis]] | [[Category: radiolysis]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:39:52 2007'' | ||
Revision as of 12:35, 30 October 2007
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STUDY ON RADIATION DAMAGE ON A CRYOCOOLED CRYSTAL. PART 4: STRUCTURE AFTER IRRADIATION WITH 27.2*10E15 PHOTONS
OverviewOverview
The high intensity of third-generation X-ray sources, along with the, development of cryo-cooling of protein crystals at temperatures around 100, K, have made it possible to extend the diffraction limit of crystals and, to reduce their size. However, even with cryo-cooled crystals, radiation, damage becomes a limiting factor. So far, the radiation damage has, manifested itself in the form of a loss of overall diffracted intensity, and an increase in the temperature factor. The structure of a protein, (myrosinase) after exposure to different doses of X-rays in the region of, 20 x 10(15) photons mm(-2) has been studied. The changes in the structure, owing to radiation damage were analysed using Fourier difference maps and, occupancy refinement for the first time. Damage was obvious in the ... [(full description)]
About this StructureAbout this Structure
1DWH is a [Protein complex] structure of sequences from [Sinapis alba] with NAG, ZN, SO4 and GOL as [ligands]. Active as [Transferred entry: 3.2.1.147], with EC number [3.2.3.1]. Structure known Active Site: ZNB. Full crystallographic information is available from [OCA].
ReferenceReference
Structural changes in a cryo-cooled protein crystal owing to radiation damage., Burmeister WP, Acta Crystallogr D Biol Crystallogr. 2000 Mar;56(Pt 3):328-41. PMID:10713520
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