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'''Unreleased structure'''


The entry 8hcn is ON HOLD  until Paper Publication
==CryoEM Structure of Klebsiella pneumoniae UreD/urease complex==
<StructureSection load='8hcn' size='340' side='right'caption='[[8hcn]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8hcn]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HCN FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hcn OCA], [https://pdbe.org/8hcn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hcn RCSB], [https://www.ebi.ac.uk/pdbsum/8hcn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hcn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0W8AWT7_KLEPN A0A0W8AWT7_KLEPN]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Urease is a nickel (Ni) enzyme that is essential for the colonization of Helicobacter pylori in the human stomach. To solve the problem of delivering the toxic Ni ion to the active site without diffusing into the cytoplasm, cells have evolved metal carrier proteins, or metallochaperones, to deliver the toxic ions to specific protein complexes. Ni delivery requires urease to form an activation complex with the urease accessory proteins UreFD and UreG. Here, we determined the cryo-electron microscopy structures of H. pylori UreFD/urease and Klebsiella pneumoniae UreD/urease complexes at 2.3- and 2.7-angstrom resolutions, respectively. Combining structural, mutagenesis, and biochemical studies, we show that the formation of the activation complex opens a 100-angstrom-long tunnel, where the Ni ion is delivered through UreFD to the active site of urease.


Authors: Nim, Y.S., Fong, I.Y.H., Deme, J., Tsang, K.L., Caesar, J., Johnson, S., Wong, K.B., Lea, S.M.
Delivering a toxic metal to the active site of urease.,Nim YS, Fong IYH, Deme J, Tsang KL, Caesar J, Johnson S, Pang LTH, Yuen NMH, Ng TLC, Choi T, Wong YYH, Lea SM, Wong KB Sci Adv. 2023 Apr 21;9(16):eadf7790. doi: 10.1126/sciadv.adf7790. Epub 2023 Apr , 21. PMID:37083535<ref>PMID:37083535</ref>


Description: CryoEM Structure of Klebsiella pneumoniae UreD/urease complex
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Fong, I.Y.H]]
<div class="pdbe-citations 8hcn" style="background-color:#fffaf0;"></div>
[[Category: Deme, J]]
== References ==
[[Category: Johnson, S]]
<references/>
[[Category: Wong, K.B]]
__TOC__
[[Category: Tsang, K.L]]
</StructureSection>
[[Category: Nim, Y.S]]
[[Category: Klebsiella pneumoniae]]
[[Category: Lea, S.M]]
[[Category: Large Structures]]
[[Category: Caesar, J]]
[[Category: Caesar J]]
[[Category: Deme J]]
[[Category: Fong IYH]]
[[Category: Johnson S]]
[[Category: Lea SM]]
[[Category: Nim YS]]
[[Category: Tsang KL]]
[[Category: Wong KB]]

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