4l71: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4l71]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vvp 1vvp], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vvq 1vvq], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vvr 1vvr] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vvs 1vvs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L71 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L71 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4l71]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vvp 1vvp], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vvq 1vvq], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vvr 1vvr] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vvs 1vvs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L71 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L71 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MG:1N-METHYLGUANOSINE-5-MONOPHOSPHATE'>1MG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=PPU:PUROMYCIN-5-MONOPHOSPHATE'>PPU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.900001Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MG:1N-METHYLGUANOSINE-5-MONOPHOSPHATE'>1MG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=PPU:PUROMYCIN-5-MONOPHOSPHATE'>PPU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l71 OCA], [https://pdbe.org/4l71 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l71 RCSB], [https://www.ebi.ac.uk/pdbsum/4l71 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l71 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l71 OCA], [https://pdbe.org/4l71 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l71 RCSB], [https://www.ebi.ac.uk/pdbsum/4l71 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l71 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
Latest revision as of 19:13, 20 September 2023
Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the RibosomeCrystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-A on the Ribosome
Structural highlights
FunctionRS2_THET8 Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B] Publication Abstract from PubMedMaintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNASufA6 and N1-methylguanosine at position 37 (m1G37) modification-deficient anticodon stem loopPro, both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNASufA6 to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m1G37 modification of tRNAPro also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by the ribosome in maintaining the correct translational reading frame. Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.,Maehigashi T, Dunkle JA, Miles SJ, Dunham CM Proc Natl Acad Sci U S A. 2014 Aug 15. pii: 201409436. PMID:25128388[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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