8f40: Difference between revisions
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==Engineering Crystals with Tunable Symmetries from 14- or 16-Base-Long DNA Strands== | |||
<StructureSection load='8f40' size='340' side='right'caption='[[8f40]], [[Resolution|resolution]] 2.45Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8f40]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8F40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8F40 FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8f40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8f40 OCA], [https://pdbe.org/8f40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8f40 RCSB], [https://www.ebi.ac.uk/pdbsum/8f40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8f40 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sequence-selective recognition of DNA duplexes is important for a wide range of applications including regulating gene expression, drug development, and genome editing. Many small molecules can bind DNA duplexes with sequence selectivity. It remains as a challenge how to reliably and conveniently obtain the detailed structural information on DNA-molecule interactions because such information is critically needed for understanding the underlying rules of DNA-molecule interactions. If those rules were understood, we could design molecules to recognize DNA duplexes with a sequence preference and intervene in related biological processes, such as disease treatment. Here, we have demonstrated that DNA crystal engineering is a potential solution. A molecule-binding DNA sequence is engineered to self-assemble into highly ordered DNA crystals. An X-ray crystallographic study of molecule-DNA cocrystals reveals the structural details on how the molecule interacts with the DNA duplex. In this approach, the DNA will serve two functions: (1) being part of the molecule to be studied and (2) forming the crystal lattice. It is conceivable that this method will be a general method for studying drug/peptide-DNA interactions. The resulting DNA crystals may also find use as separation matrices, as hosts for catalysts, and as media for material storage. | |||
Engineering DNA Crystals toward Studying DNA-Guest Molecule Interactions.,Zhang C, Zhao J, Lu B, Seeman NC, Sha R, Noinaj N, Mao C J Am Chem Soc. 2023 Mar 1;145(8):4853-4859. doi: 10.1021/jacs.3c00081. Epub 2023 , Feb 15. PMID:36791277<ref>PMID:36791277</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8f40" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Synthetic construct]] | ||
[[Category: Lu B]] | |||
[[Category: Mao C]] | |||
[[Category: Noinaj N]] | |||
[[Category: Seeman NC]] | |||
[[Category: Sha R]] | |||
[[Category: Zhang C]] | |||
[[Category: Zhao J]] | |||
Revision as of 10:36, 8 March 2023
Engineering Crystals with Tunable Symmetries from 14- or 16-Base-Long DNA StrandsEngineering Crystals with Tunable Symmetries from 14- or 16-Base-Long DNA Strands
Structural highlights
Publication Abstract from PubMedSequence-selective recognition of DNA duplexes is important for a wide range of applications including regulating gene expression, drug development, and genome editing. Many small molecules can bind DNA duplexes with sequence selectivity. It remains as a challenge how to reliably and conveniently obtain the detailed structural information on DNA-molecule interactions because such information is critically needed for understanding the underlying rules of DNA-molecule interactions. If those rules were understood, we could design molecules to recognize DNA duplexes with a sequence preference and intervene in related biological processes, such as disease treatment. Here, we have demonstrated that DNA crystal engineering is a potential solution. A molecule-binding DNA sequence is engineered to self-assemble into highly ordered DNA crystals. An X-ray crystallographic study of molecule-DNA cocrystals reveals the structural details on how the molecule interacts with the DNA duplex. In this approach, the DNA will serve two functions: (1) being part of the molecule to be studied and (2) forming the crystal lattice. It is conceivable that this method will be a general method for studying drug/peptide-DNA interactions. The resulting DNA crystals may also find use as separation matrices, as hosts for catalysts, and as media for material storage. Engineering DNA Crystals toward Studying DNA-Guest Molecule Interactions.,Zhang C, Zhao J, Lu B, Seeman NC, Sha R, Noinaj N, Mao C J Am Chem Soc. 2023 Mar 1;145(8):4853-4859. doi: 10.1021/jacs.3c00081. Epub 2023 , Feb 15. PMID:36791277[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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