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| {{STRUCTURE_1ije| PDB=1ije | SCENE= }} | | {{STRUCTURE_1ije| PDB=1ije | SCENE= }} |
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| '''Nucleotide Exchange Intermediates in the eEF1A-eEF1Ba Complex'''
| | ===Nucleotide Exchange Intermediates in the eEF1A-eEF1Ba Complex=== |
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| ==Overview==
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| In the elongation cycle of protein biosynthesis, the nucleotide exchange factor eEF1Balpha catalyzes the exchange of GDP bound to the G-protein, eEF1A, for GTP. To obtain more information about the recently solved eEF1A-eEF1Balpha structure, we determined the structures of the eEF1A-eEF1Balpha-GDP-Mg2+, eEF1A-eEF1Balpha-GDP and eEF1A-eEF1Balpha-GDPNP complexes at 3.0, 2.4 and 2.05 A resolution, respectively. Minor changes, specifically around the nucleotide binding site, in eEF1A and eEF1Balpha are consistent with in vivo data. The base, sugar and alpha-phosphate bind as in other known nucleotide G-protein complexes, whereas the beta- and gamma-phosphates are disordered. A mutation of Lys 205 in eEF1Balpha that inserts into the Mg2+ binding site of eEF1A is lethal. This together with the structures emphasizes the essential role of Mg2+ in nucleotide exchange in the eEF1A-eEF1Balpha complex.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11373622}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11373622 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11373622}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Valente, L.]] | | [[Category: Valente, L.]] |
| [[Category: Protein complex]] | | [[Category: Protein complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:03:58 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 12:19:51 2008'' |