Glycolysis Enzymes: Difference between revisions
No edit summary |
No edit summary |
||
| Line 43: | Line 43: | ||
'''Step 7: Phosphoglycerate kinase''' | '''Step 7: Phosphoglycerate kinase''' | ||
[[Shane_Harmon_Sandbox|Phosphoglycerate kinase]] catalyzes the transfer of a phosphate from the 1 position of 1,3-bisphosphoglycerate to ADP. This is the "break even" point of glycolysis: the two ATPs that were consumed in preparing for the cleavage have been now been regenerated, in addition to two molecules of NADH, which can be used to generate ATP through electron transport and oxidative phosphorylation. | [[Shane_Harmon_Sandbox|Phosphoglycerate kinase]] catalyzes the transfer of a phosphate from the 1 position of 1,3-bisphosphoglycerate to ADP. This is the "break even" point of glycolysis: the two ATPs that were consumed in preparing for the cleavage have been now been regenerated, in addition to two molecules of NADH, which can be used to generate ATP through electron transport and oxidative phosphorylation. Phosphoglycerate kinase is the seventh enzyme in the cycle which catalyzes the reaction of 1,3-Biphosphoglycerate and ADP to produce <scene name='Shane_Harmon_Sandbox/Product/2'>3-Phosphoglycerate</scene> and <scene name='Shane_Harmon_Sandbox/Atp/4'>ATP</scene>. This method for ATP production is known as substrate-level phosphorylation because it produces energy storing ATP molecules without the use of oxygen, NADH, or an ATPase. The reaction is highly exergonic allowing it to be coupled with the less thermodynamically favored GADPH reaction of the cycle so both reactions occur spontaneously. | ||
The resultant 3-phosphoglycerate isomerizes to 2-phosphoglycerate in a reaction catalyzed by [[Christopher_Vachon_Sandbox|phosphoglycerate mutase]]. A second high energy intermediate, phosphoenolpyruvate, is formed by [[Cory_Tiedeman_Sandbox_1|enolase]]. The final reaction of the pathway is catalyzed by [[Keegan_Gelvoria_Sandbox_1|pyruvate kinase]], which converts phosphoenol pyruvate to pyruvate, while generating ATP from ADP. | The resultant 3-phosphoglycerate isomerizes to 2-phosphoglycerate in a reaction catalyzed by [[Christopher_Vachon_Sandbox|phosphoglycerate mutase]]. A second high energy intermediate, phosphoenolpyruvate, is formed by [[Cory_Tiedeman_Sandbox_1|enolase]]. The final reaction of the pathway is catalyzed by [[Keegan_Gelvoria_Sandbox_1|pyruvate kinase]], which converts phosphoenol pyruvate to pyruvate, while generating ATP from ADP. | ||