4bar: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4bar]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BAR FirstGlance]. <br>
<table><tr><td colspan='2'>[[4bar]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4BAR FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EU3:EUROPIUM+(III)+ION'>EU3</scene>, <scene name='pdbligand=IKX:4-(4-(2-HYDROXYETHYL)-1H-1,2,3-TRIAZOL-1-YL)PYRIDINE-2,6-DICARBOXYLIC+ACID'>IKX</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4bal|4bal]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EU3:EUROPIUM+(III)+ION'>EU3</scene>, <scene name='pdbligand=IKX:4-(4-(2-HYDROXYETHYL)-1H-1,2,3-TRIAZOL-1-YL)PYRIDINE-2,6-DICARBOXYLIC+ACID'>IKX</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bar OCA], [https://pdbe.org/4bar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bar RCSB], [https://www.ebi.ac.uk/pdbsum/4bar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bar ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4bar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bar OCA], [https://pdbe.org/4bar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4bar RCSB], [https://www.ebi.ac.uk/pdbsum/4bar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4bar ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/THM1_THADA THM1_THADA]] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.
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<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Thaumatococcus daniellii]]
[[Category: Thaumatococcus daniellii]]
[[Category: Gautier, A]]
[[Category: Gautier A]]
[[Category: Girard, E]]
[[Category: Girard E]]
[[Category: Kahn, R]]
[[Category: Kahn R]]
[[Category: Nauton, L]]
[[Category: Nauton L]]
[[Category: Talon, R]]
[[Category: Talon R]]
[[Category: Anomalous scattering]]
[[Category: Click-chemistry]]
[[Category: De novo phasing]]
[[Category: Experimental phasing]]
[[Category: Lanthanide complex]]
[[Category: Plant protein]]

Latest revision as of 05:43, 21 November 2024

Thaumatin from Thaumatococcus daniellii structure in complex with the europium tris-hydroxyethyltriazoledipicolinate complex at 1.20 A resolution.Thaumatin from Thaumatococcus daniellii structure in complex with the europium tris-hydroxyethyltriazoledipicolinate complex at 1.20 A resolution.

Structural highlights

4bar is a 1 chain structure with sequence from Thaumatococcus daniellii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

New trisdipicolinic acid-lanthanide complexes are reported as phasing agents for X-ray crystallography of proteins. It is demonstrated that CuAAC modifications allow protein co-crystallization with low concentration of lanthanide complexes leading to an accurate structure determination.

Clicked europium dipicolinate complexes for protein X-ray structure determination.,Talon R, Nauton L, Canet JL, Kahn R, Girard E, Gautier A Chem Commun (Camb). 2012 Nov 12;48(97):11886-8. doi: 10.1039/c2cc36982f. PMID:23123834[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Talon R, Nauton L, Canet JL, Kahn R, Girard E, Gautier A. Clicked europium dipicolinate complexes for protein X-ray structure determination. Chem Commun (Camb). 2012 Nov 12;48(97):11886-8. doi: 10.1039/c2cc36982f. PMID:23123834 doi:10.1039/c2cc36982f

4bar, resolution 1.20Å

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OCA