4ac5: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4ac5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AC5 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4ac5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Blastochloris_viridis Blastochloris viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AC5 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 8.2&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCB:BACTERIOCHLOROPHYLL+B'>BCB</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MQ7:MENAQUINONE-7'>MQ7</scene>, <scene name='pdbligand=NS5:15-CIS-1,2-DIHYDRONEUROSPORENE'>NS5</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7prc|7prc]], [[1dxr|1dxr]], [[2wjm|2wjm]], [[1prc|1prc]], [[2x5v|2x5v]], [[1vrn|1vrn]], [[3prc|3prc]], [[6prc|6prc]], [[1r2c|1r2c]], [[2wjn|2wjn]], [[2x5u|2x5u]], [[5prc|5prc]], [[2prc|2prc]], [[2jbl|2jbl]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ac5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ac5 OCA], [https://pdbe.org/4ac5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ac5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ac5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ac5 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ac5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ac5 OCA], [https://pdbe.org/4ac5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ac5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ac5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ac5 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/RCEM_RHOVI RCEM_RHOVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[https://www.uniprot.org/uniprot/CYCR_RHOVI CYCR_RHOVI]] The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor. [[https://www.uniprot.org/uniprot/RCEL_RHOVI RCEL_RHOVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[https://www.uniprot.org/uniprot/RCEH_RHOVI RCEH_RHOVI]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
[https://www.uniprot.org/uniprot/CYCR_BLAVI CYCR_BLAVI] The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.<ref>PMID:10736158</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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[[Category: Blastochloris viridis]]
[[Category: Blastochloris viridis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Andreasson, J]]
[[Category: Andreasson J]]
[[Category: Aquila, A]]
[[Category: Aquila A]]
[[Category: Arnlund, D]]
[[Category: Arnlund D]]
[[Category: Bajt, S]]
[[Category: Bajt S]]
[[Category: Barthelmess, M]]
[[Category: Barthelmess M]]
[[Category: Barty, A]]
[[Category: Barty A]]
[[Category: Bogan, M J]]
[[Category: Bogan MJ]]
[[Category: Bostedt, C]]
[[Category: Bostedt C]]
[[Category: Bozek, J D]]
[[Category: Bozek JD]]
[[Category: Caleman, C]]
[[Category: Caleman C]]
[[Category: Chapman, H N]]
[[Category: Chapman HN]]
[[Category: Coffee, R]]
[[Category: Coffee R]]
[[Category: Coppola, N]]
[[Category: Coppola N]]
[[Category: Davidsson, J]]
[[Category: Davidsson J]]
[[Category: DePonte, D P]]
[[Category: DePonte DP]]
[[Category: Doak, R B]]
[[Category: Doak RB]]
[[Category: Ekeberg, T]]
[[Category: Ekeberg T]]
[[Category: Epp, S W]]
[[Category: Epp SW]]
[[Category: Erk, B]]
[[Category: Erk B]]
[[Category: Fleckenstein, H]]
[[Category: Fleckenstein H]]
[[Category: Foucar, L]]
[[Category: Foucar L]]
[[Category: Fromme, P]]
[[Category: Fromme P]]
[[Category: Graafsma, H]]
[[Category: Graafsma H]]
[[Category: Gumprecht, L]]
[[Category: Gumprecht L]]
[[Category: Hajdu, J]]
[[Category: Hajdu J]]
[[Category: Hampton, C Y]]
[[Category: Hampton CY]]
[[Category: Hartmann, A]]
[[Category: Hartmann A]]
[[Category: Hartmann, R]]
[[Category: Hartmann R]]
[[Category: Hauser, G]]
[[Category: Hauser G]]
[[Category: Hirsemann, H]]
[[Category: Hirsemann H]]
[[Category: Holl, P]]
[[Category: Holl P]]
[[Category: Hunter, M S]]
[[Category: Hunter MS]]
[[Category: Johansson, L C]]
[[Category: Johansson LC]]
[[Category: Kassemeyer, S]]
[[Category: Kassemeyer S]]
[[Category: Katona, G]]
[[Category: Katona G]]
[[Category: Kimmel, N]]
[[Category: Kimmel N]]
[[Category: Kirian, R A]]
[[Category: Kirian RA]]
[[Category: Liang, M]]
[[Category: Liang M]]
[[Category: Lomb, L]]
[[Category: Lomb L]]
[[Category: Maia, F R.N C]]
[[Category: Maia FRNC]]
[[Category: Malmerberg, E]]
[[Category: Malmerberg E]]
[[Category: Marchesini, S]]
[[Category: Marchesini S]]
[[Category: Martin, A V]]
[[Category: Martin AV]]
[[Category: Nass, K]]
[[Category: Nass K]]
[[Category: Neutze, R]]
[[Category: Neutze R]]
[[Category: Reich, C]]
[[Category: Reich C]]
[[Category: Rolles, D]]
[[Category: Rolles D]]
[[Category: Rudek, B]]
[[Category: Rudek B]]
[[Category: Rudenko, A]]
[[Category: Rudenko A]]
[[Category: Schlichting, I]]
[[Category: Schlichting I]]
[[Category: Schulz, J]]
[[Category: Schulz J]]
[[Category: Seibert, M M]]
[[Category: Seibert MM]]
[[Category: Shoeman, R L]]
[[Category: Shoeman RL]]
[[Category: Sierra, R]]
[[Category: Sierra R]]
[[Category: Soltau, H]]
[[Category: Soltau H]]
[[Category: Spence, J C.H]]
[[Category: Spence JCH]]
[[Category: Starodub, D]]
[[Category: Starodub D]]
[[Category: Stellato, F]]
[[Category: Stellato F]]
[[Category: Stern, S]]
[[Category: Stern S]]
[[Category: Struder, L]]
[[Category: Struder L]]
[[Category: Timneanu, N]]
[[Category: Timneanu N]]
[[Category: Ullrich, J]]
[[Category: Ullrich J]]
[[Category: Wahlgren, W Y]]
[[Category: Wahlgren WY]]
[[Category: Wang, X]]
[[Category: Wang X]]
[[Category: Weidenspointner, G]]
[[Category: Weidenspointner G]]
[[Category: Weierstall, U]]
[[Category: Weierstall U]]
[[Category: White, T A]]
[[Category: White TA]]
[[Category: Wunderer, C]]
[[Category: Wunderer C]]
[[Category: Cell membrane]]
[[Category: Chromophore]]
[[Category: Electron transport]]
[[Category: Formylation]]
[[Category: Lipidic-sponge phase]]
[[Category: Lipoprotein]]
[[Category: Metal- binding]]
[[Category: Photosynthesis]]
[[Category: Reaction center]]
[[Category: Transmembrane]]

Revision as of 14:23, 20 December 2023

Lipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallographyLipidic sponge phase crystal structure of the Bl. viridis reaction centre solved using serial femtosecond crystallography

Structural highlights

4ac5 is a 4 chain structure with sequence from Blastochloris viridis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 8.2Å
Ligands:, , , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CYCR_BLAVI The reaction center of purple bacteria contains a tightly bound cytochrome molecule which re-reduces the photo oxidized primary electron donor.[1]

Publication Abstract from PubMed

X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

Lipidic phase membrane protein serial femtosecond crystallography.,Johansson LC, Arnlund D, White TA, Katona G, Deponte DP, Weierstall U, Doak RB, Shoeman RL, Lomb L, Malmerberg E, Davidsson J, Nass K, Liang M, Andreasson J, Aquila A, Bajt S, Barthelmess M, Barty A, Bogan MJ, Bostedt C, Bozek JD, Caleman C, Coffee R, Coppola N, Ekeberg T, Epp SW, Erk B, Fleckenstein H, Foucar L, Graafsma H, Gumprecht L, Hajdu J, Hampton CY, Hartmann R, Hartmann A, Hauser G, Hirsemann H, Holl P, Hunter MS, Kassemeyer S, Kimmel N, Kirian RA, Maia FR, Marchesini S, Martin AV, Reich C, Rolles D, Rudek B, Rudenko A, Schlichting I, Schulz J, Seibert MM, Sierra RG, Soltau H, Starodub D, Stellato F, Stern S, Struder L, Timneanu N, Ullrich J, Wahlgren WY, Wang X, Weidenspointner G, Wunderer C, Fromme P, Chapman HN, Spence JC, Neutze R Nat Methods. 2012 Jan 29. doi: 10.1038/nmeth.1867. PMID:22286383[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Chen IP, Mathis P, Koepke J, Michel H. Uphill electron transfer in the tetraheme cytochrome subunit of the Rhodopseudomonas viridis photosynthetic reaction center: evidence from site-directed mutagenesis. Biochemistry. 2000 Apr 4;39(13):3592-602. PMID:10736158
  2. ↑ Johansson LC, Arnlund D, White TA, Katona G, Deponte DP, Weierstall U, Doak RB, Shoeman RL, Lomb L, Malmerberg E, Davidsson J, Nass K, Liang M, Andreasson J, Aquila A, Bajt S, Barthelmess M, Barty A, Bogan MJ, Bostedt C, Bozek JD, Caleman C, Coffee R, Coppola N, Ekeberg T, Epp SW, Erk B, Fleckenstein H, Foucar L, Graafsma H, Gumprecht L, Hajdu J, Hampton CY, Hartmann R, Hartmann A, Hauser G, Hirsemann H, Holl P, Hunter MS, Kassemeyer S, Kimmel N, Kirian RA, Maia FR, Marchesini S, Martin AV, Reich C, Rolles D, Rudek B, Rudenko A, Schlichting I, Schulz J, Seibert MM, Sierra RG, Soltau H, Starodub D, Stellato F, Stern S, Struder L, Timneanu N, Ullrich J, Wahlgren WY, Wang X, Weidenspointner G, Wunderer C, Fromme P, Chapman HN, Spence JC, Neutze R. Lipidic phase membrane protein serial femtosecond crystallography. Nat Methods. 2012 Jan 29. doi: 10.1038/nmeth.1867. PMID:22286383 doi:10.1038/nmeth.1867

4ac5, resolution 8.20Å

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